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Mus musculus

ABSTRACT: Single-cell RNA-seq of NR5A1-eGFP positive cells of the developing mouse testis from E10.5 to E16.5

PROVIDER: PRJNA382161 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5440940_1.fastq.gz	Fastqsanger.gz
	SRR5440940_2.fastq.gz	Fastqsanger.gz
	SRR5440950_1.fastq.gz	Fastqsanger.gz
	SRR5440950_2.fastq.gz	Fastqsanger.gz
	SRR5440960_1.fastq.gz	Fastqsanger.gz

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Single-cell RNA-seq of NR5A1-eGFP positive cells of the developing mouse testis from E10.5 to E16.5

Project description:Purpose: We performed a time-course single-cell RNA-seq of the somatic cells of the XY mouse gonads to study the cell population heterogeneity and the genetic program during their differentiation. Methods: We collected gonads from NR5A1-eGFP transgenic embryos at five embryonic stages: E10.5, E11.5, E12.5, E13.5, E16.5. Cells were captured with the C1 autoprep system and cDNA sequenced with Illumina HiSeq 2000. Results: One cell population was detected at E10.5 and gives rise to both Sertoli and fetal Leydig cells. A precursor cell population remains undifferentiated at E16.5 and are likely to be adult Leydig cell precursors. Conclusion: Our study is, to date, the most granular transcriptomic study of the developing mouse testis and provide a more complete model of somatic cell differentiation during male sex determination.

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Project description:Purpose: We performed a time-course single-cell RNA-seq of the somatic cells of the XX mouse gonads to study the cell population heterogeneity and the genetic program during their differentiation. Methods: We collected gonads from NR5A1-eGFP transgenic embryos at six embryonic stages: E10.5, E11.5, E12.5, E13.5, E16.5 and P6. Methods: Cells were capture with the C1 autoprep system and cDNA sequenced with Illumina HiSeq 2000. Results: One cell population was detected at E10.5 and give rise to both Granulosa and steroidogenic precursor cells. A precursor cell population remains undifferentiated at P6 and are likely to be theca cell precursors. Conclusion: Our study is, to date, the most granular transcriptomic study of the developing mouse ovary and provide a more complete model of somatic cell differentiation during female sex determination.

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