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Expression data from E10.5 mouse otocyst


ABSTRACT: We established a novel EGFP reporter mouse line (named Tg(ETAR-EGFP)14Imeg), which enables the placode-derived inner ear sensory cell lineage to be visualized and monitored. At E10.5, EGFP expression was detected in the ventral and dorsomedial region of the otocyst. Using this reporter line and FACS-array technology, we performed gene expression profiling focused on the regional specificity of the otocyst. We sorted E10.5 otocyst epithelial cells of heterozygous mice into EGFP-positive and EGFP-negative cells for RNA extraction and hybridization on Affymetrix microarrays. We profiled the expression of the genes in FACS-enriched cell population and conducted transcriptome analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Chisato Fujimoto 

PROVIDER: E-GEOD-19618 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Establishment of mice expressing EGFP in the placode-derived inner ear sensory cell lineage and FACS-array analysis focused on the regional specificity of the otocyst.

Fujimoto Chisato C   Ozeki Hidenori H   Uchijima Yasunobu Y   Suzukawa Keigo K   Mitani Akihisa A   Fukuhara Shigetomo S   Nishiyama Koichi K   Kurihara Yukiko Y   Kondo Kenji K   Aburatani Hiroyuki H   Kaga Kimitaka K   Yamasoba Tatsuya T   Kurihara Hiroki H  

The Journal of comparative neurology 20101201 23


In this study, we established a novel enhanced green fluorescent protein (EGFP) reporter mouse line that enables the visualization of the placode-derived inner ear sensory cell lineage. EGFP was initially expressed in the otic placode and throughout its differentiation process into the inner ear sensory patches. At embryonic day 10.5 (E10.5), EGFP was expressed in the ventral and dorsomedial region of the otocyst. These regions could mainly give rise to the cochlea, including the organ of Corti,  ...[more]

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