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Identification of glaikit in a genome-wide expression profiling for axonal bifurcation of the mushroom body in Drosophila

ABSTRACT: Identification of glaikit in a genome-wide expression profiling for axonal bifurcation of the mushroom body in Drosophila

PROVIDER: PRJNA382401 | ENA |

REPOSITORIES: ENA

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Identification of glaikit in a genome-wide expression profiling for axonal bifurcation of the mushroom body in Drosophila (white pupa)

Project description:Axonal branching is a fundamental requirement for sending electrical signals to multiple targets. However, despite the importance of axonal branching in neural development and function, the molecular mechanisms that control branch formation are poorly understood. Previous studies have hardly addressed the intracellular signaling cascade of axonal bifurcation characterized by growth cone splitting. Recently we reported that DISCO interacting protein 2 (DIP2) regulates bifurcation of mushroom body axons in Drosophila melanogaster. DIP2 mutant displays ectopic bifurcations in α/β neurons. Taking advantage of this phenomenon, we tried to identify genes involved in branching formation by comparing the transcriptome of wild type with that of DIP2 RNAi flies. After the microarray analysis, Glaikit (Gkt), a member of the phospholipase D superfamily, was identified as a downstream target of DIP2 by RNAi against gkt and qRT-PCR experiment. Single cell MARCM analysis of gkt mutant phenocopied the ectopic axonal branches observed in DIP2 mutant. Furthermore, a genetic analysis between gkt and DIP2 revealed that gkt potentially acts in parallel with DIP2. In conclusion, we identified a novel gene underlying the axonal bifurcation process.

2017-04-11 | GSE97606 | GEO

Identification of glaikit in a genome-wide expression profiling for axonal bifurcation of the mushroom body in Drosophila (29 C)

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Identification of glaikit in a genome-wide expression profiling for axonal bifurcation of the mushroom body in Drosophila (25 C)

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Identification of glaikit in a genome-wide expression profiling for axonal bifurcation of the mushroom body in Drosophila. [Agilent-021791]

Project description:Axonal branching is a fundamental requirement for sending electrical signals to multiple targets. However, despite the importance of axonal branching in neural development and function, the molecular mechanisms that control branch formation are poorly understood. Previous studies have hardly addressed the intracellular signaling cascade of axonal bifurcation characterized by growth cone splitting. Recently we reported that DISCO interacting protein 2 (DIP2) regulates bifurcation of mushroom body axons in Drosophila melanogaster. DIP2 mutant displays ectopic bifurcations in α/β neurons. Taking advantage of this phenomenon, we tried to identify genes involved in branching formation by comparing the transcriptome of wild type with that of DIP2 RNAi flies. A microarray analysis and a subsequent qRT-PCR experiment reviled that Glaikit (Gkt), a member of the phospholipase D (PLD) superfamily, is potentially a downstream target of DIP2. RNAi against gkt phenocopied the ectopic axonal branches observed in DIP2 mutant. Furthermore, a genetic analysis between gkt and DIP2 revealed that gkt acts in parallel with DIP2. In conclusion, we identified a novel gene underlying the axonal bifurcation process.

2017-02-03 | GSE94420 | GEO

Identification of glaikit in a genome-wide expression profiling for axonal bifurcation of the mushroom body in Drosophila. [Agilent-018972]

Project description:Axonal branching is a fundamental requirement for sending electrical signals to multiple targets. However, despite the importance of axonal branching in neural development and function, the molecular mechanisms that control branch formation are poorly understood. Previous studies have hardly addressed the intracellular signaling cascade of axonal bifurcation characterized by growth cone splitting. Recently we reported that DISCO interacting protein 2 (DIP2) regulates bifurcation of mushroom body axons in Drosophila melanogaster. DIP2 mutant displays ectopic bifurcations in α/β neurons. Taking advantage of this phenomenon, we tried to identify genes involved in branching formation by comparing the transcriptome of wild type with that of DIP2 RNAi flies. A microarray analysis and a subsequent qRT-PCR experiment reviled that Glaikit (Gkt), a member of the phospholipase D (PLD) superfamily, is potentially a downstream target of DIP2. RNAi against gkt phenocopied the ectopic axonal branches observed in DIP2 mutant. Furthermore, a genetic analysis between gkt and DIP2 revealed that gkt acts in parallel with DIP2. In conclusion, we identified a novel gene underlying the axonal bifurcation process.

2017-02-03 | GSE94419 | GEO

Identification of glaikit in a genome-wide expression profiling for axonal bifurcation of the mushroom body in Drosophila.

Project description:Identification of glaikit in a genome-wide expression profiling for axonal bifurcation of the mushroom body in Drosophila.

| PRJNA369656 | ENA

Mushroom Body Ethanol RNA-seq

Project description:Lasting 24hr transcriptomic response of adult Drosophila mushroom body nuclei following odors only (odors) or odors+ethanol (trained) treatment.

2018-10-25 | GSE108525 | GEO

Mushroom Body Ethanol RNA-seq

Project description:Mushroom Body Ethanol RNA-seq

| PRJNA427565 | ENA

Transcription profiling of Drosophila mushroom body neurons

Project description:Genomic analysis of axon pruning in Drosophila mushroom body neurons identifies the RNA-binding protein Boule as a negative regulator; This SuperSeries is composed of the following subset Series:; GSE10012: Timecourse: MB neurons at the onset and early steps of axon pruning; GSE10013: EcR-dependent gene expression in MB neurons at the onset of axon pruning Experiment Overall Design: Refer to individual Series

2008-06-18 | E-GEOD-10014 | biostudies-arrayexpress

Genomic analysis of axon pruning in Drosophila mushroom body neurons

Project description:Genomic analysis of axon pruning in Drosophila mushroom body neurons identifies the RNA-binding protein Boule as a negative regulator This SuperSeries is composed of the SubSeries listed below.

2008-06-01 | GSE10014 | GEO

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