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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Drosophila mushroom body neurons

ABSTRACT: Genomic analysis of axon pruning in Drosophila mushroom body neurons identifies the RNA-binding protein Boule as a negative regulator; This SuperSeries is composed of the following subset Series:; GSE10012: Timecourse: MB neurons at the onset and early steps of axon pruning; GSE10013: EcR-dependent gene expression in MB neurons at the onset of axon pruning Experiment Overall Design: Refer to individual Series

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Eric Hoopfer

PROVIDER: E-GEOD-10014 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genomic analysis of Drosophila neuronal remodeling: a role for the RNA-binding protein Boule as a negative regulator of axon pruning.

Hoopfer Eric D ED Penton Andrea A Watts Ryan J RJ Luo Liqun L

The Journal of neuroscience : the official journal of the Society for Neuroscience 20080601 24

Drosophila mushroom body (MB) gamma neurons undergo axon pruning during metamorphosis through a process of localized degeneration of specific axon branches. Developmental axon degeneration is initiated by the steroid hormone ecdysone, acting through a nuclear receptor complex composed of USP (ultraspiracle) and EcRB1 (ecdysone receptor B1) to regulate gene expression in MB gamma neurons. To identify ecdysone-dependent gene expression changes in MB gamma neurons at the onset of axon pruning, we u ...[more]

PMID: 18550751

Similar Datasets

Transcription profiling of Drosophila mushroom body neurons at the onset and early steps of axon pruning

Project description:Drosophila mushroom body (MB) Î³ neurons undergo axon pruning during metamorphosis through a process of localized degeneration of specific axon branches. Developmental axon degeneration is initiated at the onset of metamorphosis by the pre-pupal rise in the steroid hormone ecdysone. This study identifies genes that alter their expression in MB neurons at the onset and early steps of axon pruning. Experiment Overall Design: y,w animals were staged at -18, 0 and 5 hours relative to puparium formation. RNA was isolated from MB neurons labeled with mCD8::GFP driven by OK107-GAL4 by laser capture microdissection, labeled and hybridized to Affymetrix Drosophila Genome Arrays.

2008-06-18 | E-GEOD-10012 | biostudies-arrayexpress

Transcription profiling of Drosophila EcR-dependent gene expression in MB neurons at the onset of axon pruning

Project description:This study identifies genes that show EcR-dependent gene expression in MB neurons at the onset of axon pruning. Experiment Overall Design: A dominant negative form of the ecdysone receptor (EcRDN) was expressed in MB neurons by OK107-GAL4. Animals were staged at 0 and 5 hours relative to puparium formation. RNA was isolated from MB neurons labeled with mCD8::GFP driven by OK107-GAL4 by laser capture microdissection, labeled and hybridized to Affymetrix Drosophila Genome Arrays. These microarrays were compared to RNA isolated from y,w animals at similar time points.

2008-06-18 | E-GEOD-10013 | biostudies-arrayexpress

Timecourse: MB neurons at the onset and early steps of axon pruning

Project description:Drosophila mushroom body (MB) γ neurons undergo axon pruning during metamorphosis through a process of localized degeneration of specific axon branches. Developmental axon degeneration is initiated at the onset of metamorphosis by the pre-pupal rise in the steroid hormone ecdysone. This study identifies genes that alter their expression in MB neurons at the onset and early steps of axon pruning. Keywords: timecourse

2008-06-01 | GSE10012 | GEO

RNA-seq analysis of gene expression associated to altered gene dosage of DeIF6 during eye development in Drosophila melanogaster

Project description:We investigated the transcriptome of the eye imaginal discs and retinae in wild type (GMR/+) and DeIF6 overexpressing (GMR>DeIF6) flies at the third instar larva stage and pupa 40 hours after puparium formation.

2019-07-18 | E-MTAB-5954 | biostudies-arrayexpress

Actin dependent astrocytic infiltration is a key step for axon defasciculation during remodeling

Project description:A strocytes are essential for synapse formation, maturation and plasticity, yet their function during developmental neuronal remodeling is largely unknown To identify astrocytic molecules required for axon pruning of mushroom body ( γ neurons in Drosophi la we profile d the expression of astrocytes before ( and after ( remodeling. F ocus ing on genes enriched in larval astrocytes we identified 12 genes that are required in astrocytes, for axon pruning including the F actin regulat ors Arpc1 and form3 Interestingly, perturbing astrocytic actin dynamics did not affect their gross morphology migration or TGF β secretion In contrast, actin dynamics was required for astrocyte infiltration into the axon bundle at the onset of pruning. Remarkably, decreasing axonal adhesion facilitated the infiltration of Arpc1 KD astrocytes, and suppressed the pruning defect driven by the astrocytic perturbation Together, our findings suggest that actin dependent astrocytic infiltration is a key step in axon pruning thus promoting our understanding of neuron glia interactions during remodeling

2023-02-12 | GSE190743 | GEO

EcR-dependent gene expression in MB neurons at the onset of axon pruning

Project description:This study identifies genes that show EcR-dependent gene expression in MB neurons at the onset of axon pruning. Keywords: nuclear receptor gene regulation

2008-06-01 | GSE10013 | GEO

Genomic analysis of axon pruning in Drosophila mushroom body neurons

Project description:Genomic analysis of axon pruning in Drosophila mushroom body neurons identifies the RNA-binding protein Boule as a negative regulator This SuperSeries is composed of the SubSeries listed below.

2008-06-01 | GSE10014 | GEO

Mushroom body cell type comparison

Project description:The Drosophila melanogaster mushroom bodies (MBs) are brain structures critical for olfactory memory. The approximately 2000 intrinsic MB neurons are divisible into alpha/beta neurons, alpha'/beta' neurons and gamma neurons by morphology and roles in memory processing. It has also been shown that the different subtypes of MB neurons have different function in the process of memory formation. This difference in function, suggested to us that different types of MB neurons might have different expression profiles. In this study, we used cell-type specific gene expression profiling to gain insight into cellular properties of MB neurons.

2013-01-10 | E-MEXP-3798 | biostudies-arrayexpress

Genomic analysis of axon pruning in Drosophila mushroom body neurons

Project description:Genomic analysis of axon pruning in Drosophila mushroom body neurons

| PRJNA108199 | ENA

Transcription profiling of w118 Drosophila melanogaster sampled at multiple developmental stages to dentifies genes that alter their expression in synchrony with the late third instar and prepupal pulses of 20

Project description:This study identifies genes that alter their expression in synchrony with the late third instar and prepupal pulses of 20E. Experiment Overall Design: RNA was isolated from w1118 whole animals staged at -18, -4, 0, 2, 4, 6, 8, 10, and 12 hours relative to pupariation, labeled, and hybridized to Affymetrix Drosophila Genome Arrays.

2007-10-31 | E-GEOD-3057 | biostudies-arrayexpress

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