Dataset Information

Piscirickettsia salmonis LF-89 = ATCC VR-1361 isolate:Lf-89

ABSTRACT: Piscirickettsia salmonis LF-89 = ATCC VR-1361 isolate:Lf-89 Raw sequence reads

PROVIDER: PRJNA383157 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5456200_1.fastq.gz	Fastqsanger.gz
	SRR5456200_2.fastq.gz	Fastqsanger.gz
	SRR5456199_1.fastq.gz	Fastqsanger.gz
	SRR5456199_2.fastq.gz	Fastqsanger.gz

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Project description:Fuentealb2016 - Genome-scale metabolic reconstruction (iPF215) of Piscirickettsia salmonis LF-89 This model is described in the article: Genome-scale metabolic reconstruction for the insidious bacterium in aquaculture Piscirickettsia salmonis. Fuentealba P, Aros C, Latorre Y, Martínez I, Marshall S, Ferrer P, Albiol J, Altamirano C. Bioresour. Technol. 2017 Jan; 223: 105-114 Abstract: Piscirickettsia salmonis is a fish bacterium that causes the disease piscirickettsiosis in salmonids. This pathology is partially controlled by vaccines. The lack of knowledge has hindered its culture on laboratory and industrial scale. The study describes the metabolic phenotype of P. salmonis in culture. This study presents the first genome-scale model (iPF215) of the LF-89 strain of P. salmonis, describing the central metabolic pathway, biosynthesis and molecule degradation and transport mechanisms. The model was adjusted with experiment data, allowing the identification of the capacities that were not predicted by the automatic annotation of the genome sequences. The iPF215 model is comprised of 417 metabolites, 445 reactions and 215 genes, was used to reproduce the growth of P. salmonis (?max 0.052±0.005h(-1)). The metabolic reconstruction of the P. salmonis LF-89 strain obtained in this research provides a baseline that describes the metabolic capacities of the bacterium and is the basis for developing improvements to its cultivation for vaccine formulation. This model is hosted on BioModels Database and identified by: MODEL1610250000. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Dataset Information

Piscirickettsia salmonis LF-89 = ATCC VR-1361 isolate:Lf-89

Dataset's files

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