Project description:Cultured NIH/3T3 cels were infeced at MOI of 10 with wild type and RDR mutant of SFV virus.. Total RNA was extracted 8 hours post-infection for gene expression analysis.
Project description:Swiss murine embryonic fibroblasts (NIH-3T3) were infected with wild-type Smith strain mouse cytomegalovirus (MCMV) at a multiplicity of infection (MOI) of 10. Sequencing libraries were prepared using the cRNA-seq protocol from the indicated time points of infection as described by Whisnant A.W. et al, Nat. Commun (2020)
Project description:Swiss murine embryonic fibroblasts (NIH-3T3) were infected with wild-type Smith strain mouse cytomegalovirus (MCMV) at a multiplicity of infection (MOI) of 10. Sequencing libraries were prepared using the 4sU-seq protocol (Dölken et al., RNA 2008 and Rutkowski et al.) from the indicated time points of infection as described in Rutkowski, A.J. et al, Nat. Commun (2015)
Project description:Analysis of Immediate Early Response 2 (Ier2)-inducible NIH 3T3 cells after Ier2 induction with RheoSwitch ligand RSL-1. Results provide insight into the function of Ier2 in NIH 3T3 mouse embryonal fibroblasts. Immediate early genes, including Ier2, are rapidly induced in quiescent cells by proliferation and migration-inducing stimuli. Microarray gene expression profiling was performed to identify differentially expressed genes following overexpression of Ier2 in NIH 3T3-Ier2 inducible cells after 24 hour induction of Ier2.
Project description:In this study, we compared the transcriptional profile of NIH-3T3 cells that are either wild-type (WT) or Nrf1 knockout (Nrf1-KO). We treated these cells with carfilzomib for 6 hours or 24 hours and performed RNA-sequencing with these samples.
Project description:Swiss murine embryonic fibroblasts (NIH-3T3) were infected with wild-type Smith strain mouse cytomegalovirus (MCMV) at a multiplicity of infection (MOI) of 10. Ribosome profiling was performed at various times during infection with minor modification to the protocol described in Stern-Ginossar N et al., Science 2012. The detailed protocol is described in Rutkowski et al, Nature commun 2015, and Whisnant et al., Nature commun. 2020. Translation start site profiling was performed by culturing cells in presence of Harringtonin or Lactimidomycin for 30 min prior to cell harvest.
Project description:Swiss murine embryonic fibroblasts (NIH-3T3) were infected with wild-type Smith strain mouse cytomegalovirus (MCMV) at a multiplicity of infection (MOI) of 10. Metabolic RNA labelling was performed using 400µM 4sU for one hour prior to cell lysis. Total RNA was isolated using the Trizol protocol and U-to-C conversions were induced by IAA treatment according to the SLAM-seq protocol (Herzog et al., Nature Methods 2017). Sequencing libraries were prepared using the dRNA-seq protocol from the indicated time points of infection as described by Whisnant A.W. et al, Nat. Commun (2020).
Project description:The purpose is to obtain samples for mRNA analysis in primary mouse myeloid dendritic cells infected with wild type West Nile virus (WNVMT) and mutant virus (WNVE218A).
Project description:The purpose is to obtain samples for mRNA analysis in primary mouse myeloid dendritic cells infected with wild type West Nile virus (WNVMT) and mutant virus (WNVE218A).
Project description:NIH-3T3 cells were infected with wildtype MCMV at a multiplicity of infection (MOI) of 10. Ribosome profiling was performed at the indicated times of infection as described in Rutkowski et al., Nature Communications 2015.
