Project description:We performed microarray analysis and real-time quantitative PCR (RT-qPCR) to detect the expression levels of lncRNA in blood collected from 2 WT patients. Goal was to determine the underlying mechanism by which lncRNA regulates WT progression.
Project description:CHART sequencing has been carried out in order to evaluate SCIRT target genes in MDA-MB-231 sphere derived cells. We discovered that lncRNA SCIRT binds promoter region of genes whose expression changes after SCIRT siRNA downregulation, suggesting that those are target SCIRT target genes.
Project description:This study was conducted by using Flag tag to IP proteins expressed with fusion small peptides encoding LncRNA MAGI2-AS3 and identified by high resolution mass spectrometry.
Project description:To explore the potential target lncRNAs of EZH2 in breast cancer cells, we determined the lncRNA expression profiles in MCF7-control and MCF7-EZH2 overexpressing cells using lncRNA Microarray.
Project description:LncRNA RPPH1 has been reported to be highly expressed in a variety of cancers. In order to determine the function and mechanism of lncRNA RPPH1 in breast cancer, we overexpressed lncRNA RPPH1 in breast cancer MDA-MB-231 cells, and analyzed the gene expression changes in these RPPH1 overexpressed cells compared with the control cells by RNA-seq.
Project description:To identify biologically and clinically novel lncRNAs potentially involved in the progression of breast cancer, we profiled the expression of lncRNAs in two stage III triple-negativebreast cancer tissues and their paired adjacent noncancerous tissues by LncRNA Array 3.0 (ArrayStar). Expression of the mostly upregulated lncRNA (BCAR4) from this signature was quantified in the breast caner tissue microarray by RNA In situ Hybridization and bioinformatic analysis of Oncomine database, confirming its correlation with breast cancer metastasis.
