Project description:We analyze high-throughput profiling of histone H3K36me3 in mouse embryonic stem cells from chromatin immunoprecipitated DNA captured by H3K36me3-specific antibody. We find that H3K36me3 distribution is like expected and enriched at transcribing gene bodies especially with enrichment at 3’-terminal of gene body, which is identical with findings observed in other mammalian cells.
Project description:We adopt histone H3 mutants competitive incorporation system to screen the effects of them on genetic fidelity. We find incorporation of H3K36M, an oncogenic histone mutant, produce a cancer or ageing-like genetic landscape by punctuating the H3K36me3/H3K9me3 dependent DNA repair mechanism. This work firstly get a direct relationship between epigenetic inheritance and genomic stability
Project description:Copy numbers of mouse cells (ESCs and NIH3T3) carrying a series of single-site histone mutants or shRNAs comparing with control empty cells. Genomic DNA from candidate cells and control cells were respectively labeled by Cy5 or Cy3 and analyzed by array-based comparative genomic hybridization (aCGH).
Project description:The goals of this project are to study the transcriptome profiling (RNA-seq) of mouse embryonic stem cells by histone mutants incorporation (H3.1K3M, H3.1K36M vs H3.1wt) or shRNA-mediated knockdown of methyl-transferase Suv39h1/h2 (shSuv39h1, shSuv39h2 vs shNC)
Project description:This SuperSeries is composed of the following subset Series: GSE24646: h3k36me3-Establishing a reference epigenome in arabidopsis seedlings GSE24647: h3k9me2-Establishing a reference epigenome in arabidopsis seedlings GSE24648: h3k4me2-Establishing a reference epigenome in arabidopsis seedlings GSE24649: h3k4me3-Establishing a reference epigenome in arabidopsis seedlings GSE24650: h3k9me3-Establishing a reference epigenome in arabidopsis seedlings GSE24651: h3k27me3-Establishing a reference epigenome in arabidopsis seedlings GSE24652: h3k27me2-Establishing a reference epigenome in arabidopsis seedlings GSE24653: h3k56ac-Establishing a reference epigenome in arabidopsis seedlings GSE24654: h4k20me1-Establishing a reference epigenome in arabidopsis seedlings GSE24655: h3-Establishing a reference epigenome in arabidopsis seedlings GSE24656: h2bub-Establishing a reference epigenome in arabidopsis seedlings GSE24657: h3k27me3_gw-Establishing a reference epigenome in arabidopsis seedlings GSE24658: h3k4me3_gw-Establishing a reference epigenome in arabidopsis seedlings GSE24659: h3k4me2_gw-Establishing a reference epigenome in arabidopsis seedlings GSE24660: 5mc_gw-Establishing a reference epigenome in arabidopsis seedlings GSE24661: h3k36me3_gw-Establishing a reference epigenome in arabidopsis seedlings GSE24662: h3k27me1_gw-Establishing a reference epigenome in arabidopsis seedlings GSE24663: h3_gw-Establishing a reference epigenome in arabidopsis seedlings GSE24664: h3k27me3_roots_gw-Establishing a reference epigenome in arabidopsis seedlings GSE24665: h3k4me3_roots_gw-Establishing a reference epigenome in arabidopsis seedlings Refer to individual Series