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Mus musculus

ABSTRACT: Transcriptome analysis of C2C12 cells (WT and MYOD KO) overexpressing MUNC: proliferating and differentiating conditions.

PROVIDER: PRJNA387733 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5595180_1.fastq.gz	Fastqsanger.gz
	SRR5595180_2.fastq.gz	Fastqsanger.gz
	SRR5595190_1.fastq.gz	Fastqsanger.gz
	SRR5595190_2.fastq.gz	Fastqsanger.gz
	SRR5595181_1.fastq.gz	Fastqsanger.gz

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MUNC downregulation decreases muscle differentiation

Project description:Gene expression profiling in differentiating C2C12 cells comparing control cells and MUNC-deprived cells RNA samples were isolated from 4 C2C12 samples: proliferating control (siC) cells, differentiating siC cells, proliferating and differentiating MUNC depleted (siMUNC) cells

2014-11-27 | E-GEOD-63673 | biostudies-arrayexpress

Transcriptome analysis of C2C12 cells (WT and MYOD KO) overexpressing MUNC: proliferating and differentiating conditions.

Project description:To check global changes of genes expression correlating with induction of MUNC, we stably overexpressed MUNC in WT cells and in MYOD KO cells. We compared transcriptomes of control cells and cells overexpressing MUNC and distinguished genes that are differentially expressed in different conditions

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miR-487b, miR-3963 and miR-6412 delayed myogenic differentiation in C2C12 mouse myoblast-derived cells.

Project description:We newly identified skeletal muscle differentiation-associated miRNAs by comparing miRNA expression profile between C2C12 cell and Wnt4-overexpressing C2C12 cell. miR-487b, miR-3963 and miR-6412 are significantly down-regulated in differentiating C2C12 cells, and transfection of their mimics resulted in reduced expression of myogenic differentiation markers including Troponin T, myosin heavy chain fast and slow type. Single analysis for each condition (proliferating C2C12 cells, differentiating C2C12 cells, proliferating Wnt4-overexpressing C2C12 subline cells

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Project description:Wnt/beta-catenin signaling is involved in various aspects of skeletal muscle development and regeneration. Using C2C12 cells, we examined intracellular signaling and gene transcription during myoblast proliferation and differentiation. The results of the present studies suggest that Wnt signaling is interacting with TGF-beta superfamily signaling through Smad activation. Single analysis for each condition (proliferating C2C12 cells, differentiating C2C12 cells, proliferating Wnt4-overexpressing C2C12 subline cells).

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Murine postnatal subventricular zone (SVZ) neural stem cells (NSCs): Wild-type (WT) vs. Dnmt3a-null (KO)

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