Ontology highlight
ABSTRACT:
PROVIDER: PRJNA388016 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:isolation of Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH), partial cds in Nigella sativa
| PRJDB13838 | ENA
Project description:Aerial parts (AP) and roots of wild-type plants were compared with plastidial glyceraldehyde-3-phosphate dehydrogenase double mutants (gapcp1gapcp2). These mutants were also compared with conditional mutants after GAPCp induction.
2015-10-30 | E-MTAB-3262 | biostudies-arrayexpress
Project description:Glycolytic Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyzes the conversion of glyceraldehyde 3-phospate to 1,3-bisphosphoglycerate by coupling with the reduction of NAD+ to NADH. Both cytosolic and plastidial isoforms of GAPDH has been described but the in vivo functions of the plastidial isoforms is unresolved. We generated mutants of the Arabidopsis plastidial GAPDH isoforms (At1g79530, At1g16300; GAPCp1, GAPCp2) and performed a microarray analysis comparing gapcp double (gapcp1 gapcp2) mutant and wild type seedlings
2009-02-10 | GSE14765 | GEO
Project description:Non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) is a conserved protein found in higher plants. In photosynthetic cells, the enzyme is involved in a shuttle transfer mechanism to export NADPH from the chloroplast to the cytosol. In this work, we demonstrate that GAPN gene express in leaves and roots at similar levels; showing the highest level of expression in flowers. To investigate the role of this enzyme in different plant tissues, we characterized a mutant from Arabidopsis thaliana having an insertion at the GAPN gene locus. The homozygous mutant was determined to be null respect to GAPN, as it exhibited complete absence of both expression of GAPN mRNA and enzyme activity. Transcriptome analysis demonstrated that the insertion mutant plant shows altered expression of several enzymes involved in carbohydrate metabolism. Significantly, cytosolic phosphorylating (NAD-dependent) glyceraldehyde-3-phosphate dehydrogenase mRNA levels are induced in the mutant, which correlates with an increase in enzyme activity. mRNA levels and enzymatic activity of glucose-6-phosphate dehydrogenase were also elevated, correlating with an increase in NADPH concentration. Moreover, an increased levels of oxidative stress was measured in the mutant plants. Downregulation of several glycolytic and photosynthetic genes suggests that GAPN is important for the efficiency of both metabolic processes. The results presented demonstrate that GAPN has a relevant role in plant growth and development. Keywords: 2 color experiment in triplicate including dye-swap
2005-11-23 | GSE3539 | GEO
Project description:Glycolytic Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyzes the conversion of glyceraldehyde 3-phospate to 1,3-bisphosphoglycerate by coupling with the reduction of NAD+ to NADH. Both cytosolic and plastidial isoforms of GAPDH has been described but the in vivo functions of the plastidial isoforms is unresolved. We generated mutants of the Arabidopsis plastidial GAPDH isoforms (At1g79530, At1g16300; GAPCp1, GAPCp2) and performed a microarray analysis comparing gapcp double (gapcp1 gapcp2) mutant and wild type seedlings Experiment Overall Design: 15-day old Arabidopsis seedlings (Col 0) and gapcp1gapcp2 double mutants were used for RNA extraction and hybridization on Affymetrix microarrays.
2009-02-21 | E-GEOD-14765 | biostudies-arrayexpress
Project description:Glycolytic Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyzes the conversion of glyceraldehyde 3-phospate to 1,3-bisphosphoglycerate by coupling with the reduction of NAD+ to NADH. We generated mutants of the Arabidopsis plastidial GAPDH isoforms (At1g79530, At1g16300; GAPCp1, GAPCp2). gapcp double mutants (gapcp1 gapcp2) display a drastic phenotype of arrested root development and sterility.Complex interactions occurring between ABA and sugar signal transduction pathways have been shown, but the molecular mechanisms connecting both pathways are not well understood. Since we found drastic carbohydrate changes in gapcp1 gapcp2, we studied their response to ABA. by performing a microarray analysis comparing gapcp1 gapcp2 and wild type seedlings after a long term treatment with ABA.
2010-05-11 | GSE21765 | GEO
Project description:Mathematical Definition
symbol/abbreviation
Mathematical symbols
Keq Equilibrium constant
Ki Inhibition constant
Km Affinity constant
v Predicted enzyme activities
Vf Maximal enzyme activities under saturating substrate and activator
conditions, and in the absence of inhibitors
h Hill coefficient
k Rate constant
Abbreviations
ACET Acetaldehyde
ADH Alcohol dehydrogenase
AK Adenylate kinase
ATPcons ATP consuming reactions
bPG 1,3-bisphosphoglycerate
ENO Enolase
ETOHcy Cytoplasmic ethanol
ETOHex Extracellular ethanol
ETOHexp Ethanol transport
GAP Glyceraldehyde 3-phosphate
GAPD Glyceraldehyde 3-phosphate dehydrogenase
GF Glucose facilitator
GK Glucokinase
GLUCcy Cytoplasmic glucose
GLUCex Extracellular glucose
GLUC6P Glucose 6-phosphate
GPD Glucose 6-phosphate dehydrogenase
KDPG 2-keto-3-deoxy-6-phosphogluconate
KDPGA 2-keto-3-deoxy-6-phosphogluconate aldolase
PDC Pyruvate decarboxylase
PEP Phosphoenolpyruvate
PGD 6-phosphogluconate dehydratase
PGK 3-phosphoglycerate kinase
PGL 6-phosphogluconolactonase
PGLACTON 6-phosphogluconolactone
PGLUCONATE 6-phosphogluconate
PGM Phosphoglycerate mutase
P3G 3-phosphoglycerate
P2G 2-phosphoglycerate
PYK Pyruvate kinase
PYR Pyruvate
2020-08-06 | MODEL2008060001 | BioModels
Project description:The model corresponds to the third columns (model simulations) in tables 3 and 5 of publication (PMID: 24085837, DOI: 10.1099/mic.0.071340-0)
Mathematical Definition
symbol/abbreviation
Mathematical symbols
Keq Equilibrium constant
Ki Inhibition constant
Km Affinity constant
v Predicted enzyme activities
Vf Maximal enzyme activities under saturating substrate and activator
conditions, and in the absence of inhibitors
h Hill coefficient
k Rate constant
Abbreviations
ACET Acetaldehyde
ADH Alcohol dehydrogenase
AK Adenylate kinase
ATPcons ATP consuming reactions
bPG 1,3-bisphosphoglycerate
ENO Enolase
ETOHcy Cytoplasmic ethanol
ETOHex Extracellular ethanol
ETOHexp Ethanol transport
GAP Glyceraldehyde 3-phosphate
GAPD Glyceraldehyde 3-phosphate dehydrogenase
GF Glucose facilitator
GK Glucokinase
GLUCcy Cytoplasmic glucose
GLUCex Extracellular glucose
GLUC6P Glucose 6-phosphate
GPD Glucose 6-phosphate dehydrogenase
KDPG 2-keto-3-deoxy-6-phosphogluconate
KDPGA 2-keto-3-deoxy-6-phosphogluconate aldolase
PDC Pyruvate decarboxylase
PEP Phosphoenolpyruvate
PGD 6-phosphogluconate dehydratase
PGK 3-phosphoglycerate kinase
PGL 6-phosphogluconolactonase
PGLACTON 6-phosphogluconolactone
PGLUCONATE 6-phosphogluconate
PGM Phosphoglycerate mutase
P3G 3-phosphoglycerate
P2G 2-phosphoglycerate
PYK Pyruvate kinase
PYR Pyruvate
2014-09-15 | MODEL1409050000 | BioModels
Project description:The model corresponds to the Table 4 and the last column of table 5 of publication (PMID: 24085837, DOI: 10.1099/mic.0.071340-0).
Mathematical Definition
symbol/abbreviation
Mathematical symbols
Keq Equilibrium constant
Ki Inhibition constant
Km Affinity constant
v Predicted enzyme activities
Vf Maximal enzyme activities under saturating substrate and activator
conditions, and in the absence of inhibitors
h Hill coefficient
k Rate constant
Abbreviations
ACET Acetaldehyde
ADH Alcohol dehydrogenase
AK Adenylate kinase
ATPcons ATP consuming reactions
bPG 1,3-bisphosphoglycerate
ENO Enolase
ETOHcy Cytoplasmic ethanol
ETOHex Extracellular ethanol
ETOHexp Ethanol transport
GAP Glyceraldehyde 3-phosphate
GAPD Glyceraldehyde 3-phosphate dehydrogenase
GF Glucose facilitator
GK Glucokinase
GLUCcy Cytoplasmic glucose
GLUCex Extracellular glucose
GLUC6P Glucose 6-phosphate
GPD Glucose 6-phosphate dehydrogenase
KDPG 2-keto-3-deoxy-6-phosphogluconate
KDPGA 2-keto-3-deoxy-6-phosphogluconate aldolase
PDC Pyruvate decarboxylase
PEP Phosphoenolpyruvate
PGD 6-phosphogluconate dehydratase
PGK 3-phosphoglycerate kinase
PGL 6-phosphogluconolactonase
PGLACTON 6-phosphogluconolactone
PGLUCONATE 6-phosphogluconate
PGM Phosphoglycerate mutase
P3G 3-phosphoglycerate
P2G 2-phosphoglycerate
PYK Pyruvate kinase
PYR Pyruvate
2014-09-15 | MODEL1409050001 | BioModels