Project description:We performed differential RNA-sequencing (dRNA-seq) experiments in both minimal medium (MM) plus β-hydroxybutyrate (β-HB) and MM plus tetralin (THN) of Sphingopyxis granuli strain TFA. The objective was mapping the Transcription Start Site (TSS) of each gene in the genome in both conditions, detecting non-coding RNAs (ncRNAs) and comparing the gene expression profile in a preferential carbon source (β-HB) versus tetralin (an aromatic pollutant). The dRNA-seq technique consists of using a termination exonuclease (TEX) to allow the discrimination of primary and processed transcripts. Furthermore, to detect Hfq-bound RNAs we co-immunoprecipitated RNA from the wild type strain (negative control) and a TFA strain with an Hfq-3xFlag tagged version (MPO501 strain) using an anti-3xFlag antibody and performed RNA-sequencing from the precipitated RNA.

2018-08-14 | GSE111181 | GEO

Sphingopyxis lindanitolerans strain:WS5A3p

Project description:Sphingopyxis lindanitolerans strain:WS5A3p Genome sequencing and assembly

| PRJNA418918 | ENA

Sphingopyxis soli strain:BL03

Project description:Sphingopyxis soli strain:BL03 Genome sequencing and assembly

| PRJNA707349 | ENA

Sphingopyxis lutea strain:DHUNG17

Project description:Sphingopyxis lutea strain:DHUNG17 Genome sequencing and assembly

| PRJNA689945 | ENA

Sphingopyxis solisilvae strain:R366

Project description:Sphingopyxis solisilvae strain:R366 Genome sequencing and assembly