Project description:Saos-2-2, a TSG101-deficient stable line, was dereived from Saos-2 cells. The goal of this experiment was to determine the effects of TSG101 down-regulation on global gene expression.
Project description:Saos-2-2, a TSG101-deficient stable line, was dereived from Saos-2 cells. The goal of this experiment was to determine the effects of TSG101 down-regulation on global gene expression. Saoa-2 and Saos-2-2 samples were labeled with Cys-3 and Cys-5 respectively, and hybrided with microarray simutaneously to differentiate the up- and down-regulated genes.
Project description:We performed DNase-seq on two osteosarcoma cell lines, Saos-2 and U2OS. This was done to identify regulatory regions within the genomes of these cells. We performed hotspot calling on these data using Hotspot2 (https://github.com/Altius/hotspot2) to identify 51,556 DNaseI hypersensitive sites within Saos-2 cells and 63,388 within U2OS cells.
