Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

119 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Saos-2 vs. Saos-2-2 cells

ABSTRACT: Saos-2-2, a TSG101-deficient stable line, was dereived from Saos-2 cells. The goal of this experiment was to determine the effects of TSG101 down-regulation on global gene expression. Saoa-2 and Saos-2-2 samples were labeled with Cys-3 and Cys-5 respectively, and hybrided with microarray simutaneously to differentiate the up- and down-regulated genes.

ORGANISM(S): Homo sapiens

SUBMITTER: Tzu-Hao Cheng

PROVIDER: E-GEOD-50808 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

RNAi profiling of human SAOS-2 cells, RBP2 knockdown or pRB overexpression

Project description:RBP2 is downstream of pRB pathway; We used gene expression profiling experiments to investigate if gene expression changes in cells with RBP2 knockdown significantly overlap with gene expression changes in cells overexpressing pRB, consistent with the data that knockdown of RBP2 phenocopies reintroduction of pRB in SAOS-2 (RB-/-) cells Experiment Overall Design: Knockdown of RBP2 by siRNA to RBP2 (RBP2siRNA) in SAOS-2 (RB-/-) cells in comparison to overexpression of pRB in the SOAS-2 (RB-/-) cells and scrambled version of the RBP2 siRNA (RBP2scsiRNA)

2008-10-25 | E-GEOD-9690 | biostudies-arrayexpress

Identification of target genes regulated by M-NM-^TNp63M-NM-1 in SaOS-2 cells stably expressed with M-NM-^TNp63M-NM-1

Project description:To identify the target gene of M-NM-^TNp63M-NM-1, in osteosarcoma cells, we performed the global gene expression profiling in SaOS- 2 cells stably overexpressing the M-NM-^TNp63M-NM-1 gene (SaOS- 2-M-NM-^TNp63M-NM-1). M-NM-^TNp63M-NM-1 target genes were identified by comparison with genes expressed from SaOS-2-EV transfected with an empty vector ( SaOS-2-EV). Total RNA was extracted from SaOS- 2-M-NM-^TNp63M-NM-1 and SaOS-2-EV cells cultured in three independent replicates for each condition. Cy3-labeled cRNA was synthesized and hybridized to Agilent human 8 x 60K microarrays. Raw data, obtained after array image analysis were normalized and used to retrieve differentially expressed genes in M-NM-^TNp63M-NM-1 overexpressing SaOS- 2 cells and genes from control SaOS-2-EV cells.

2014-02-27 | E-GEOD-54942 | biostudies-arrayexpress

Saos-2 vs. Saos-2-2 cells

Project description:Saos-2-2, a TSG101-deficient stable line, was dereived from Saos-2 cells. The goal of this experiment was to determine the effects of TSG101 down-regulation on global gene expression.

2013-10-11 | GSE50808 | GEO

Comparison of gene expression in human sarcoma cell line SaOS-2 cells between differentiation and maintenance culture

Project description:We cultured human sarcoma cell line SaOS-2 with or without differentiation and compared gene expression patterns between both cultures by microarray. The experiments were either in triplicate or duplicate.

2016-02-07 | E-GEOD-71677 | biostudies-arrayexpress

Affymetrix CytoscanHD data for genomic plasticity of osteosarcoma cell lines

Project description:To identify chromosomal aberrations during passaging of parental SAOS-2 osteosarcoma cells with low metastatic potential and of SAOS-2-derived LM5 cells with increased metastatic potential DNA of early and late passage SAOS-2 and LM5 cells was analyzed by Affymetrix Cytoscan HD genotyping arrays

2015-05-01 | E-GEOD-67125 | biostudies-arrayexpress

The effects of SMYD2 overexpression on ectopic RB in SAOS cells

Project description:Expression analysis of wild-type SAOS cells and SAOS cells transiently transfected with RB, SMYD2, or RB and SMYD2.

2011-01-01 | GSE22842 | GEO

Genome-wide analyses of XRN1-sensitive targets in osteosarcoma cells identifies disease-relevant transcripts containing G-rich motifs

Project description:RNA-seq of ribosome depleted RNA extracted from SAOS-2 cells following treatment with either XRN1 siRNA or a scrambled siRNA control

2021-08-16 | E-MTAB-10808 | biostudies-arrayexpress

Identification of target genes regulated by ΔNp63α in SaOS-2 cells stably expressed with ΔNp63α

Project description:To identify the target gene of ΔNp63α, in osteosarcoma cells, we performed the global gene expression profiling in SaOS- 2 cells stably overexpressing the ΔNp63α gene (SaOS- 2-ΔNp63α). ΔNp63α target genes were identified by comparison with genes expressed from SaOS-2-EV transfected with an empty vector ( SaOS-2-EV).

2014-02-27 | GSE54942 | GEO

Transcriptome analysis of HeLa cells upon deprivation of methionine and cysteine (Met/Cys)

Project description:We have previously shown that deprivation of essential amino acids, namely methionine/cysteine or tyrosine, leads to the transcriptional reactivation of integrated silenced transgenes, and latent HIV-1 provirus. In an effort to understand the underlying mechanisms, here we investigate the overall transcriptional profile of HeLa cells upon Met/Cys starvation for different time points, including the expression of repeated and transposable elements. HeLa cells carrying a stably integrated and silenced plasmid expressing the OA1/GPR143 gene (pcDNA3.1-OA1) were cultured in regular DMEM medium for 6-30-120 hours, or in absence of Met/Cys for 6-15-30-72-120 hours, and the changes in the expression of genes and repeated elements was evaluated by RNAseq

2018-07-21 | E-MTAB-6452 | biostudies-arrayexpress

DNase-seq profiling of Saos-2 and U2OS cell lines

Project description:We performed DNase-seq on two osteosarcoma cell lines, Saos-2 and U2OS. This was done to identify regulatory regions within the genomes of these cells. We performed hotspot calling on these data using Hotspot2 (https://github.com/Altius/hotspot2) to identify 51,556 DNaseI hypersensitive sites within Saos-2 cells and 63,388 within U2OS cells.

2020-01-03 | GSE142160 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data