Project description:Whole-genome microarray gene expression profiling of primary human monocytes and B-cells cultured at high density. Monocytes and B-cells were isolated from PMBCs of 2 healthy donors and cultured at 1x10^7 cells/mL (high density). Samples were harvested for microarray gene expresssion profiling at 0, 2, 10 or 24hrs (monocytes) or 0 or 24hrs (B-cells). Protein expression of FcgR2b is higher on monocytes in high density conditions compared to low density (1x10^6 cells/mL) conditions where expression is negligble. This study provides information on genome-wide gene expression changes that occur in high density culture in order to study associations with FcgR2b expression. B-cells cultured at high density and low density show similar levels of moderate FcgR2b expression. B cells at 0hr and after 24hr of high density culture are included in this dataset as a comparators.
Project description:Increased survival of primary B-CLL cells cultured in high cell density: Evidence for activation of pathways also induced by stromal cells.
Project description:ATAC sequencing of primary human monocytes cultured at low and high density. Monocytes isolated from PBMCs of 3 healthy donors were cultured at low density (1 x 10^6 cells/mL) or at high density (1 x10^7 cells/mL) for 24hrs and harvested for ATAC sequencing. Protein expression of FcgR2b is higher on monocytes in high density conditions compared to low density conditions, where expression is negligble. This study provides information on genome-wide chromatin accessibility changes that occur in high density culture in order to study associations with FcgR2b expression.
Project description:CD14+ cells in the PBMCs of CLL patients are converted into NLCs. CD14+ PBMCs from healthy donors when cultured with CLL cells give rise to differentiated cells called CD14CLL-cells, which are immunophenotypically similar to NLCs. CD14+ PBMCs from healthy donors when cultured with non-malignant B cells give rise to differentiated cells called CD14B-cells. Transcriptome wide analysis reveals genes involved in immune response to be significantly deregulated between CD14B-cells and CD14CLL-cells/NLCs.
Project description:Increased survival of primary B-CLL cells cultured in high cell density: Evidence for activation of pathways also induced by stromal cells. Expression profiles of B-CLL cells before and after three different survival-inducing culture conditions: (1) stromal cell coculture [HCo], (2) stromal cell conditioned medium [CM], (3) high cell density cultures [CD]. Controls without survival-inducing culture conditions are included [0]. Peripheral blood samples were obtained from B-CLL patients.
Project description:CD14+ cells in the PBMCs of CLL patients are converted into NLCs. CD14+ PBMCs from healthy donors when cultured with CLL cells give rise to differentiated cells called CD14CLL-cells, which are immunophenotypically similar to NLCs. CD14+ PBMCs from healthy donors when cultured with non-malignant B cells give rise to differentiated cells called CD14B-cells. Transcriptome wide analysis reveals genes involved in immune response to be significantly deregulated between CD14B-cells and CD14CLL-cells/NLCs. Total RNA obtained from NLCs, CD14CLL-cells and CD14B-cells and hybridised to arrays (4 samples in each group)
Project description:Genome-wide profiling of DNA methylation 35 kb upstream and 5 kb downstream of microRNAs in 24 CLL patients and B cells of 2 healthy donors versus a pool of 10 healthy donors.
Project description:Genome-wide profiling of DNA methylation 35 kb upstream and 5 kb downstream of microRNAs in 24 CLL patients and B cells of 2 healthy donors versus a pool of 10 healthy donors. 26 samples total (24 CLL B cell samples and 2 healthy donor B cell samples) were hybridized against a pool of 10 healthy donor B cell samples.
Project description:We analyzed small RNA sequencing data from CD5+/CD19+ B cells of a cohort of indolent and aggressive CLL patients compared with CD19+ B-cells of healthy donors. We identified tsRNA signatures in indolent and aggressive CLL vs. normal B-cells; we also found a drastic dysregulation of the expression of mature tRFs in CLL.
