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Microarray timecourse profiling of gene expression changes in primary human monocytes and B-cells cultured at high density to investigate FcgR2b associated gene expression changes

ABSTRACT: Whole-genome microarray gene expression profiling of primary human monocytes and B-cells cultured at high density. Monocytes and B-cells were isolated from PMBCs of 2 healthy donors and cultured at 1x10^7 cells/mL (high density). Samples were harvested for microarray gene expresssion profiling at 0, 2, 10 or 24hrs (monocytes) or 0 or 24hrs (B-cells). Protein expression of FcgR2b is higher on monocytes in high density conditions compared to low density (1x10^6 cells/mL) conditions where expression is negligble. This study provides information on genome-wide gene expression changes that occur in high density culture in order to study associations with FcgR2b expression. B-cells cultured at high density and low density show similar levels of moderate FcgR2b expression. B cells at 0hr and after 24hr of high density culture are included in this dataset as a comparators.

ORGANISM(S): Homo sapiens

PROVIDER: GSE165643 | GEO | 2022/04/13

REPOSITORIES: GEO

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