Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA394115 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR5822300.fastq.gz | Fastqsanger.gz | |||
| SRR5822292.fastq.gz | Fastqsanger.gz | |||
| SRR5822293.fastq.gz | Fastqsanger.gz | |||
| SRR5822294.fastq.gz | Fastqsanger.gz | |||
| SRR5822295.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 9 |
Similar Datasets
Project description:Purpose: Several animal models are available for investigating retinal vein occlusion (RVO). Laser-induced RVO has been studied in pigs, rabbits, and rats. Mice have been rarely used despite the huge number of available mutants, ease of handling and cost effectiveness. The aim of this study was to find out if the RVO mouse model is useful for investigating effects of hypoxia. Methods: C57Bl/6J mice were injected with eosin Y for photo-sensitization. Subsequently, large retinal veins were laser-treated in one eye to induce vascular occlusion. Contralateral control eyes received non-occlusive laser treatment of the retina sparing large vessels. The animals were followed for up to eight days and assessed by fundoscopy, angiography, hypoxyprobe staining, histopathology and gene expression analysis by qPCR and RNA sequencing. Another group of mice was left untreated and studied at a single time point to determine baseline characteristics. Results: In the RVO group, half of the retinal veins stayed occluded for three days, and one third did so for the whole test period of 8 days. Hypoxia was observed in all RVO eyes for up to 5 days with a maximum around days 2-3. Large retinal edematous areas were present in all laser-treated eyes irrespective of vascular occlusion status. Accordingly, expression of genes associated with inflammation or cell damage was highly up-regulated in both groups as compared to untreated eyes while expression of genes associated with angiogenesis or hypoxia did not change much. Conclusion: In the laser-induced RVO mouse model, general tissue damage and inflammation responses predominate and obscure specific hypoxia- or angiogenesis-related changes. A non-occlusive control laser treatment is essential to allow for proper data interpretation.
2018-03-14 | GSE101398 | GEO
Project description:Retinal ischemia is believed to initiate a series of events that leads to retinal neovascularization observed in several retinal diseases such as diabetetic retinopathy and retinal vein occlusion. Although some molecules such as VEGF and MCP-1 have been implicated in the process, its underlying mechanisms remain elusive. In order to identify genes associated with retinal ischemia, we performed gene expression analyses in retinas of mouse model of oxygen-induced retinopathy using DNA microarray technology.
2010-01-15 | GSE19886 | GEO
Project description:Intraocular pressure was elevated through episkleral vein occlusion by thermic cauterization of SD rats. Animals were further sacrificed after different periods of elevated IOP and the retinal proteins were investigated for alterations regarding the relative protein level in this experimental model of glaucoma.
2017-11-28 | MSV000081757 | MassIVE
Project description:Branch retinal vein occlusion (BRVO) is a common retinal vascular disease, but global protein changes following the disease remain largely unelucidated. To bring new insights into pathological processes and elucidate potential therapeutic targets, large-scale retinal protein changes following BRVO were studied by combining a porcine model of experimental BRVO with proteomic analysis by label-free liquid chromatography mass spectrometry.
2015-07-30 | PXD001800 | Pride
Project description:In mammals, retinal damage is followed by Müller glia cell activation and proliferation. While retinal gliosis persists in adult mammals after an insult or disease, some vertebrates, including zebrafish, have the capacity to regenerate. We believe we are the first group to show that gliosis is a fibrotic-like process in mammals’ eyes caused by differential activation of canonical and non-canonical TGFβ signaling pathways.
2019-11-03 | GSE132140 | GEO
Project description:Introgressed variants from other species can be an important source of genetic variation because they may arise rapidly, can include multiple mutations on a single haplotype, and have often been pretested by selection in the species of origin. Although introgressed alleles are generally deleterious, several studies have reported introgression as the source of adaptive alleles-including the rodenticide-resistant variant of Vkorc1 that introgressed from Mus spretus into European populations of Mus musculus domesticus. Here, we conducted bidirectional genome scans to characterize introgressed regions into one wild population of M. spretus from Spain and three wild populations of M. m. domesticus from France, Germany, and Iran. Despite the fact that these species show considerable intrinsic postzygotic reproductive isolation, introgression was observed in all individuals, including in the M. musculus reference genome (GRCm38). Mus spretus individuals had a greater proportion of introgression compared with M. m. domesticus, and within M. m. domesticus, the proportion of introgression decreased with geographic distance from the area of sympatry. Introgression was observed on all autosomes for both species, but not on the X-chromosome in M. m. domesticus, consistent with known X-linked hybrid sterility and inviability genes that have been mapped to the M. spretus X-chromosome. Tract lengths were generally short with a few outliers of up to 2.7 Mb. Interestingly, the longest introgressed tracts were in olfactory receptor regions, and introgressed tracts were significantly enriched for olfactory receptor genes in both species, suggesting that introgression may be a source of functional novelty even between species with high barriers to gene flow.
| S-EPMC8784167 | biostudies-literature
Project description:Intraocular pressure was elevated through episkleral vein occlusion by thermic cauterization of SD rats. Animals were further sacrificed after different periods of elevated IOP and the retinal proteins were investigated for alterations regarding the relative protein level in this experimental model of glaucoma.
2017-04-11 | PXD005258 | Pride
Project description:We collected whole genome testis expression data from hybrid zone mice. We integrated GWAS mapping of testis expression traits and low testis weight to gain insight into the genetic basis of hybrid male sterility.
2014-12-04 | GSE61417 | GEO
Project description:Primary objectives: It is the primary objective of this study to assess whether there is a change and a correlation between ocular blood flow parameters (retinal vessel diameters, choroidal blood flow, fundus pulsation amplitude, retinal blood velocity and thickness) and treatment response in mCRC treated with standard of care anti-angiogenic regimens in combination with chemotherapy.
Primary endpoints: Retinal vessel diameters using The Retinal Vessel AnalyzerChoroidal blood flow using Laser Doppler flowmetryRetinal blood velocity by laser Doppler velocimeterFundus pulsation amplitude using laser interferometryRetinal thickness by OCT Plasma level of sVEGFR, VEGF, Tie2, intraocular pressure
| 2532658 | ecrin-mdr-crc