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Role of BET proteins in YAP/TAZ-dependent transcription

ABSTRACT: Role of BET proteins in YAP/TAZ-dependent transcription

PROVIDER: PRJNA397706 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5919380.fastq.gz	Fastqsanger.gz
	SRR5919390.fastq.gz	Fastqsanger.gz
	SRR5919400.fastq.gz	Fastqsanger.gz
	SRR5919410.fastq.gz	Fastqsanger.gz
	SRR5919420.fastq.gz	Fastqsanger.gz

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Role of BET proteins in YAP/TAZ-dependent transcription [ChIP-seq]

Project description:We performed ChIP-seq experiments to investigate the role of BET proteins in YAP/TAZ transcriptional activity.

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Characterization of the interactomes of YAP and TAZ in podocytes identifies a relation between YAP and TAZ and proteins of the nuclear shuttling machinery

Project description:The two effector proteins of the Hippo signaling pathway, YAP and TAZ, play a pivotal role in the cellular homeostasis of podocytes and in the pathogenesis of focal segmental glomerulosclerosis (FSGS). We aim to unravel the unique and redundant functions of YAP and TAZ in the podocyte by identifying podocyte-specific interactors. We generated stable heat sensitive mouse podocytes (hsMPs) carrying a single copy integration of a transgenic construct expressing a flagged version of mouse Yap (3XFLAG.YAP), Taz (3XFLAG.TAZ) or Ruby (3XFLAG.RUBY) in the Rosa26 locus. To explore the interactome of YAP and TAZ in podocytes we immunoprecipitated the tagged proteins and characterized the co-immunoprecipitated protein complexes by mass spectrometry. Within the interactome analyses of the hsMPs, we identified shared and non-shared interacting proteins between YAP and TAZ. Among these identified proteins many well established interactors of YAP and TAZ were included, like proteins of the Tead family, different angiomotins or large tumor suppressor kinase 1 (Lats1). Strikingly, among the shared proteins were numerous proteins of the nuclear shuttling machinery, like importins (Ipo), exportins (Xpo), transportins (Tnpo) and nucleoporins (Nup) that form the nuclear pore complex (NPC), such as NUP107, NUP133, NUP205 and XPO5.

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Yap and Taz in Neural Crest play a Crucial Role in Smooth Muscle Development

Project description:We conditionally knocked out both Yap and Taz in cranial neural crest (CNC) using the Wnt1Cre driver and sequenced mRNA from embryonic day 10.5 mandibles. Examination of mRNA level in E10.5 mandibular tissues from control and Wnt1Cre Taz and Yap dKO mutant.

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YAP/TAZ-dependent transcriptome in cholangiocarcinoma cells

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