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Trypanosoma brucei

ABSTRACT: Profiling of T. brucei rRNA Nm landscape using RibOxi-seq

PROVIDER: PRJNA397920 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5926290_1.fastq.gz	Fastqsanger.gz
	SRR5926290_2.fastq.gz	Fastqsanger.gz
	SRR5926300_1.fastq.gz	Fastqsanger.gz
	SRR5926300_2.fastq.gz	Fastqsanger.gz
	SRR5926291_1.fastq.gz	Fastqsanger.gz

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Mapping of 2'-O-methylation in Trypanosoma brucei small RNAs using RibOxi-seq

Project description:In this study we combined small RNA isolation from post-ribosomal supernatant with RibOxi-seq to identify 2’-O-methylation (Nm) in single nucleotide resolution. Our analysis identified ~49 Nm sites in tRNA, snRNAs, snoRNAs, 7SL RNA and vtRNA.

2024-05-01 | GSE186967 | GEO

High-throughput mapping of 2'-O-methylation in Trypanosoma brucei poly(A) RNAs using RibOxi-seq

Project description:In this study we used RibOxi-seq to identify 2’-O-methylation (Nm) present on mRNAs in single nucleotide resolution. Our analysis identified hundreds of Nm sites in mRNAs, predominantly in CDS and 3’ UTR.

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RBP9-TAP RIPseq in Trypanosoma brucei

Project description:RBP9-TAP was expressed in bloodstream T. brucei and bound mRNA was pulled down using IgG beads and eluted via TEV-protease cleavage. rRNA was depleted using RNAseH and rRNA hybridizing oligos.

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Project description:Trypanosoma brucei were isolated from cattle, in Bunya, Uganda (Tb065BAPC) or Apuru, Uganda (Tb098AAPC). Parasites were stored as stabilities after a single mouse passage. After a further mouse passage the parasites were grown in rats to the parasitaemias indicated, isolated on DEAE cellulose and RNA was prepared. We also compared rRNA depletion with poly(A) selection.

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Project description:T. brucei PF cells were treated with several chemical reagents and anti-trypanosomatid drugs. The effect of each chemical perturbation on the transcriptome of T. brucei was examined by transcript profiling of treated vs. control cells. The results indicated widespread changes, suggesting that the transcriptome of T. brucei is highly responsive to environmental factors that perturb its metabolic and biological pathways.

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Digital Gene Expression data of a group 1 Trypanosoma brucei gambiense vs a Trypanosoma brucei brucei

Project description:Trypanosoma brucei gambiense is the causative agent of the fatal human disease African sleeping sickness. Using Digital Gene Expression we have compared the transcriptome of a group 1 T.b.gambiense (Eliane) and a T.b.brucei (STIB 247).

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Trypanosoma brucei transcriptome changes upon exposure to the aminomethyl-benzoxaborole AN3057

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Project description:Mapping of 2'-O-methylation in Trypanosoma brucei small RNAs using RibOxi-seq

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Project description:Trypanosoma brucei brucei Raw sequence reads

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