Anopheles gambiae
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA399852 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Proteomic analysis of Anopheles gambiae brain tissue after in-gel trypsin digestion. To gain insights into neurobiology of the Anopheles gambiae mosquito, we carried out a proteomic analysis of its brain using a comprehensive proteomic approach.
2016-12-20 | PXD000630 | Pride
Project description:We custom-built a bioinformatics pipeline to search for 20E-modifying enzymes in the accessory glands of Anopheles gambiae males, searching for ecdysteroid kinases (EcK), ecdysone oxidases (EO), and ecdysteroid-phosphate phosphatases (EPP). To this end, we generated RNAseq datasets of different An. gambiae tissues dissected from virgin and mated females and males, and produced similar datasets for Anopheles albimanus, a South American species that does not synthetize and transfer ecdysteroids during mating. These analyses led to the identification of one candidate EPP and two potential EcKs (EcK1 and EcK2), which we demonstrated are involved in the activity of a male-specific oxidized ecdysteroid (3D20E). We further determined that 3D20E is specifically produced by the An. gambiae male accessory glands and is transferred to females during copulation, where it triggers a series of post-mating responses.
2022-04-20 | GSE198665 | GEO
Project description:The transcriptional profile of four tissues for the multi insecticide Anopheles gambiae (Tiassale) and lab susceptible Anopheles gambiae strain N'Gousso. The malpighian tubules, abodmen integument (containing the fat body epidermal, neuronal, muscle and oenocyte cells), midgut and remaining structures were dissected and compared two ways: (i) each body part against the corresponding whole organism (ii) resistant against corresponding susceptible body parts.
2014-08-29 | E-MTAB-2808 | biostudies-arrayexpress
Project description:Anopheles gambiae mosquitoes transmit the human malaria parasite Plasmodium falciparum, which causes the majority of fatal malaria cases worldwide. The hematophagous life style defines the mosquito reproductive biology and is exploited by P. falciparum for its own sexual reproduction and transmission. The two main phases of the mosquito reproductive cycle, pre-vitellogenic (PV) and post-blood meal (PBM) shape its capacity to transmit malaria. Transition between these phases is tightly coordinated to ensure homeostasis between mosquito tissues and successful reproduction. One layer of control is provided by microRNAs, well-known regulators of blood meal digestion and egg development in mosquitoes. Here, we report a global overview of tissue-specific miRNA expression during the PV and PBM phases and identify miRNAs regulated during PV to PBM transition. The observed coordinated changes in the expression levels of a set of miRNAs in the energy-storing tissues suggest a role in the regulation of blood meal-induced metabolic changes.
2017-08-25 | GSE103034 | GEO
Project description:Whole genome transcription was quantified in adult female and male Anopheles gambiae atdifferent ages; 0 (0-24 h), 10, 20 and 30 days post-eclosion. The objective of the experiment was to identify genes with significant age-dependent transcription.
2010-01-01 | GSE18194 | GEO
Project description:Expression profiling of different adult female tissues isolated from Anopheles gambiae females
| PRJNA120051 | ENA
Project description:Anopheles gambiae Transcriptome or Gene expression
| PRJNA272547 | ENA
Project description:Anopheles gambiae Transcriptome or Gene expression
| PRJNA272536 | ENA
Project description:The age of mosquitoes is a crucial determinant of their susceptibility to infection, probability of survival to transmit pathogens and tolerance to insecticides. We investigated changes to the abundance of proteins found in heads and thoraces of Anopheles gambiae and Anopheles stephensi as they aged. Protein expression changes were assessed using two-dimensional difference gel electrophoresis and the identity of differentially expressed proteins was determined by using either matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry or capillary high-pressure liquid chromatography coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometer. Protein biomarkers were validated by quantitative Western blot analysis.
2015-08-26 | PXD002153 | Pride