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Mus musculus

ABSTRACT: Transcriptomic analysis of in vitro cultured horizontal basal cells of the murine olfactory epithelium

PROVIDER: PRJNA401939 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6011950_1.fastq.gz	Fastqsanger.gz
	SRR6011950_2.fastq.gz	Fastqsanger.gz
	SRR6011951_1.fastq.gz	Fastqsanger.gz
	SRR6011951_2.fastq.gz	Fastqsanger.gz
	SRR6011952_1.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of in vitro cultured horizontal basal cells of the murine olfactory epithelium

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Project description:Wild-type horizontal basal cells (HBCs) from mouse olfactory epithelium were profiled using single-cell ATAC-seq (10x Genomics v2) to identify regions of accessible chromatin in individual cells before and 24 hours after acute injury.

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Following the p63/Keratin5 Basal Cells in the Sensory and Nonsensory Epithelia of The Vomeronasal Organ

Project description:The Vomeronasal organ (VNO) is a part of the accessory olfactory system, which is responsible for detecting pheromones, chemical factors that trigger a spectrum of sexual and social behaviors. The vomeronasal epithelium (VNE) shares several features with the epithelium of the main olfactory epithelium (MOE). However, it is a distinct neuroepithelium populated by chemosensory neurons that differ from the olfactory sensory neurons (OSNs) in cellular structure, receptor expression, and connectivity. The vomeronasal organ of rodents comprises a sensory epithelium and a thin nonsensory epithelium that morphologically resembles the respiratory epithelium. Sox2-positive cells have been previously identified as the stem cell population that gives rise to neuronal progenitors in MOE and VNE. In addition to these, the MOE also comprises p63 positive horizontal basal cells (HBCs), a second pool of quiescent stem cells that become active in response to injury. Immunolabeling against the transcription factor p63, Keratin-5 (Krt5), Krt14 and Krt5Cre tracing experiments highlighted the existence of horizontal basal cells distributed along the basal lamina of the VNO forming from progenitors along the basal lamina oft the marginal zones. Moreover, these experiments revealed that the NSE of rodents is, like the respiratory epithelium, a stratified epithelium where the p63/Krt5+ basal cells self-replicate and give rise to the apical columnar cells facing the lumen of the VNO.

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Project description:The lineage of wild-type horizontal basal cells (HBC) stem cells from the olfactory epithelium were profiled by single-cell RNA-Seq (10X v3 chemistry) to identify differences in aged versus not-aged adult stem cells mRNA expression profiles

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Project description:The lineage of the horizontal basal cells (HBC) stem cells and their descendants from the olfactory epithelium were profiled by single-cell RNA-Seq to identify differentiated cell types, intermediate stages, transition states, and to infer the lineage trajectories.

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Mouse Olfactory Epithelium, Lineage-Traced Injury timecourse K5-Cre ER^T2 > Rosa YFP

Project description:The lineage of wild-type horizontal basal cells (HBC) stem cells from the olfactory epithelium were profiled by single-cell RNA-Seq (10X v2 chemistry) to identify differentiated cells types, intermediate stages, transition states, and to infer the lineage trajectories.

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