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Growth factor-free, chemically-defined culture system for expansion and derivation of human pluripotent stem cells

ABSTRACT: Growth factor-free, chemically-defined culture system for expansion and derivation of human pluripotent stem cells

PROVIDER: PRJNA403994 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5121900_1.fastq.gz	Fastqsanger.gz
	SRR5121900_2.fastq.gz	Fastqsanger.gz
	SRR5121901_1.fastq.gz	Fastqsanger.gz
	SRR5121901_2.fastq.gz	Fastqsanger.gz
	SRR5121902_1.fastq.gz	Fastqsanger.gz

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Project description:Cardiomyocytes can be differentiated from human pluripotent stem cells (hPSCs) in defined conditions, but efficient and consistent cardiomyocyte differentiation often requires expensive reagents such as B27 supplement or recombinant albumin. Using a chemically defined albumin-free (E8 basal) medium, we identified heparin as a novel factor that significantly promotes cardiomyocyte differentiation efficiency, and developed an efficient method to differentiate hPSCs into cardiomyocytes. The treatment of heparin helped cardiomyocyte differentiation consistently reach at least 80% purity (up to 95%) from more than 10 different hPSC lines in chemically defined DMEM/F-12 based medium on either Matrigel or defined matrices like Vitronectin and Synthemax. One of heparinâs main functions was to act as a WNT modulator that helped promote robust and consistent cardiomyocyte production. Our study provides an efficient, reliable, and cost-effective method for cardiomyocyte derivation from hPSCs that can be used for potential large-scale drug screening, disease modeling, and future cellular therapies. 12 human pluripotent stem cells (hPSCs) at three different cardiac differentiation times (0 Days, 3 Days, 6 Days, 10 Days) under different culture conditions (+/- Heparin, +/- IWP2).

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Growth factor-free, chemically-defined culture system for expansion and derivation of human pluripotent stem cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-09-10 | GSE103669 | GEO

Heparin promotes cardiac differentiation of human pluripotent stem cells in chemically defined albumin-free medium enabling consistent manufacture of cardiomyocytes

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