Project description:We performed single-cell transcriptome analysis with the 10X Genomic Chromium platform of CD4+ T cells infiltrating human primary and metastatic colorectal cancer (CRC) and non-small-cell lung cancer (NSCLC). Datasets derive from fresh samples collected and immediately processed by cell sorting and single-cell isolation 10X protocol. A total number of 10000 cells per sample was analyzed by scRNA-seq.
Project description:8 spatial RNA-seq experiments (10x Genomics Visium) and 5 single-cell RNA-seq experiments (10x Genomics Chromium) using high-grade serous ovarian carcinoma (HGSOC) samples obtained from the ovaries during the interval debulking surgery
Project description:The Merkel cell carcinoma cell line (MCC) WaGa was sequenced using the 10x Genomics 3' Chromium v2.0 platform in order to analyse transcriptional heterogeneity on a single cell level
Project description:We thus isolated hippocampus from rhesus macaques and performed single-cell RNA-sequencing analysis. The scRNA-Seq libraries were generated using the 10X Genomics Chromium Controller Instrument and Chromium Single Cell 3’ V2 Reagent Kits (10X Genomics, Pleasanton, USA).
Project description:The Merkel cell carcinoma cell line (MCC) WaGa was sequenced without and under HDAC inhibitor treatment using the 10x Genomics 3' Chromium v2.0 platform. The treatment induced changes were analysed on a single cell level.
Project description:To understand the diversity of expression states in cancer cell lines, we profiled 198 cancer cell lines by single-cell RNA-seq with 10X chromium.
Project description:Normal adjacent tissues from colorectal cancer patients were collected for MAIT cell sorting. Sorted MAIT cells from seven individual donors were pooled together. Single-cell RNA sequencing libraries were prepared using the Chromium 5' Single Cell Gene Expression Kit (10x Genomics), following the manufacturer's protocol. Sequencing was performed on a NextSeq 500 platform (Illumina).