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Myxococcus xanthus

ABSTRACT: Predatory mechanisms in co-culture of M.xanthus and E.coli Transcriptome

PROVIDER: PRJNA408275 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6314001_1.fastq.gz	Fastqsanger.gz
	SRR6314001_2.fastq.gz	Fastqsanger.gz
	SRR6314002_1.fastq.gz	Fastqsanger.gz
	SRR6314002_2.fastq.gz	Fastqsanger.gz
	SRR6314023_1.fastq.gz	Fastqsanger.gz

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Genetic components of Escherichia coli involved in its complex preypredator interaction with Myxococcus xanthus

Project description:Myxococcus xanthus and Escherichia coli represent a well-studied microbial predatorprey pair frequently examined in laboratory settings. While significant progress has been made in comprehending the mechanisms governing M. xanthus predation, various aspects of the response and defensive mechanisms of E. coli as prey remain elusive. In this study, the E. coli large-scale chromosome deletion library was screened, and a mutant designated as ME5012 was identified to possess remarkable resistance to predation by M. xanthus. Within the deleted region of ME5012 encompassing seven genes, the significance of dusB and fis genes in driving the observed resistant phenotype became apparent. Specifically, the deletion of fis resulted in a notable reduction in flagellum production in E. coli, contributing to a certain level of resistance against predation by M. xanthus. Meanwhile, the removal of dusB in E. coli led to diminished inducibility of myxovirescin A production by M. xanthus, accompanied by a slight increase in resistance to myxovirescin A. These findings shed light on the molecular mechanisms underlying the complex interaction between M. xanthus and E. coli in a predatory context.

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