Project description:Here we applied a novel approach to isolate nuclei from complex plant tissues (https://doi.org/10.1371/journal.pone.0251149), to dissect the transcriptome profiling of the hybrid poplar (Populus tremula × alba) vegetative shoot apex at single-cell resolution.
Project description:Illumina technology was used to generate mRNA profiles of a time course of Laccaria bicolor S238N and Populus tremula x alba 717-1B4 in vitro ectomycorrhizal development. Total RNA was extracted, TruSeq mRNA Stranded libraries were constructed and and sequenced in triplicates (2 x 150 bp Illumina HiSeq3000) at the Genotoul sequencing facilities (Toulouse, France). Raw reads were trimmed for low quality (quality score 0.05), Illumina adapters and sequences shorter than 15 nucleotides and aligned to the L. bicolor v2 reference transcripts available at the JGI database https://mycocosm.jgi.doe.gov/Lacbi2/Lacbi2.home.html using CLC Genomics Workbench v8.
Project description:The Populus homeobox gene ARBORKNOX1 was overexpressed behind the cauliflower mosaic virus 35S promoter in clone INRA 717 P. alba X P. tremula. The experiment here compares gene expression in defoliated stems of two independently transformed clones (mutants) versus wild type controls. Keywords: mutant versus wildtype
Project description:The Populus homeobox gene ARBORKNOX2 was overexpressed behind the cauliflower mosaic virus 35S promoter in clone INRA 717 P. alba X P. tremula. The experiment here compares gene expression in defoliated stems of two independently transformed clones (mutants) versus wild type controls. Keywords: mutant versus wildtype