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Cell-type specific sequencing of microRNAs from complex animal tissues I

ABSTRACT: Cell-type specific sequencing of microRNAs from complex animal tissues I

OTHER RELATED OMICS DATASETS IN: GSE104470GSE104466

PROVIDER: PRJNA412474 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6112540.fastq.gz	Fastqsanger.gz
	SRR6112550.fastq.gz	Fastqsanger.gz
	SRR6112560.fastq.gz	Fastqsanger.gz
	SRR6112570.fastq.gz	Fastqsanger.gz
	SRR6112580.fastq.gz	Fastqsanger.gz

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Project description:MicroRNAs (miRNAs) play an essential role in the post-transcriptional regulation of animal development and physiology. However, in vivo studies linking miRNA-function to the biology of distinct cell types within complex tissues remain challenging, partly due to the difficulties in achieving cellular resolution using available miRNA-profiling methods. Here, we report an in vivo small RNA-tagging approach that enables high-throughput sequencing of tissue- and cell type-specific miRNAs in animals, which we call microRNome by methylation-dependent sequencing, or mime-seq. The method combines orthogonal, cell-type-specific 3´ terminal 2’-O-methylation of animal miRNAs by the plant-specific methyltransferase HEN1, with oxidation-sensitive small RNA cloning and high-throughput sequencing. We show that mime-seq uncovers the miRNomes of rare cells within C. elegans and Drosophila at unprecedented specificity and sensitivity, enabling miRNA profiling with single-cell resolution in whole animals. Mime-seq overcomes the current challenges in tissue- and cell-type-specific small RNA profiling and provides novel entry points for understanding the function of miRNAs in spatially restricted physiological settings.

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