Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cell-type specific sequencing of microRNAs from complex animal tissues IV

ABSTRACT: Cell-type specific sequencing of microRNAs from complex animal tissues IV

OTHER RELATED OMICS DATASETS IN: GSE104470GSE104466

PROVIDER: PRJNA412782 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6123200.fastq.gz	Fastqsanger.gz
	SRR6123201.fastq.gz	Fastqsanger.gz
	SRR6123199.fastq.gz	Fastqsanger.gz

Items per page:

1 - 3 of 3

Similar Datasets

Cell-type specific sequencing of microRNAs from complex animal tissues

Project description:Cell-type specific sequencing of microRNAs from complex animal tissues

| PRJNA412780 | ENA

Cell-type specific sequencing of microRNAs from complex animal tissues IV

Project description:MicroRNAs (miRNAs) play an essential role in the post-transcriptional regulation of animal development and physiology. However, in vivo studies linking miRNA-function to the biology of distinct cell types within complex tissues remain challenging, partly due to the difficulties in achieving cellular resolution using available miRNA-profiling methods. Here, we report an in vivo small RNA-tagging approach that enables high-throughput sequencing of tissue- and cell type-specific miRNAs in animals, which we call microRNome by methylation-dependent sequencing, or mime-seq. The method combines orthogonal, cell-type-specific 3´ terminal 2’-O-methylation of animal miRNAs by the plant-specific methyltransferase HEN1, with oxidation-sensitive small RNA cloning and high-throughput sequencing. We show that mime-seq uncovers the miRNomes of rare cells within C. elegans and Drosophila at unprecedented specificity and sensitivity, enabling miRNA profiling with single-cell resolution in whole animals. Mime-seq overcomes the current challenges in tissue- and cell-type-specific small RNA profiling and provides novel entry points for understanding the function of miRNAs in spatially restricted physiological settings.

2018-02-14 | GSE104469 | GEO

Cell-type specific sequencing of microRNAs from complex animal tissues III

Project description:Cell-type specific sequencing of microRNAs from complex animal tissues III

| PRJNA412778 | ENA

Cell-type specific sequencing of microRNAs from complex animal tissues I

Project description:Cell-type specific sequencing of microRNAs from complex animal tissues I

| PRJNA412474 | ENA

Cell-type specific sequencing of microRNAs from complex animal tissues V

Project description:Cell-type specific sequencing of microRNAs from complex animal tissues V

| PRJNA419385 | ENA

Cell-type specific sequencing of microRNAs from complex animal tissues II

Project description:Cell-type specific sequencing of microRNAs from complex animal tissues II