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Genome-wide profiling of circular RNAs in rapidly growing culms of moso bamboo

ABSTRACT: Genome-wide profiling of circular RNAs in rapidly growing culms of moso bamboo

PROVIDER: PRJNA414226 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6171240_1.fastq.gz	Fastqsanger.gz
	SRR6171240_2.fastq.gz	Fastqsanger.gz
	SRR6171250_1.fastq.gz	Fastqsanger.gz
	SRR6171250_2.fastq.gz	Fastqsanger.gz
	SRR6171241_1.fastq.gz	Fastqsanger.gz

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Genome-wide profiling of circular RNAs in rapidly growing culms of moso bamboo

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Project description:Bamboo represents the only major lineage of grasses that is native to forests and is one of the most important non-timber forest products in the world. Moso bamboo is a large woody bamboo that has ecological, economic and cultural value in Asia and accounts for ~70% of the total bamboo growth area (Peng et al., 2013). In the aspect of epigenetics of Moso bamboo,the total genomic DNA methylation rates in Moso bamboo at different chronological ages were significantly different (Yuan et al., 2014). Those show that the flowering of Moso bamboo are closely related to epigenetic modification. However, DNA methylation in single base resolution has never been reported in moso bamboo. In this study, leaves from three-week bamboo, one-year bamboo, flower in next year bamboo, flowering bamboo and Flower florets was used for bisulfite sequencing (BS-seq), and RNA-Seq. Genome-wide methylation profile and gene expression analysis were constructed to reveal the factors to regualte the phase transition from vegetative to reproductive growth in moso bamboo.

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Project description:Moso bamboo is a fast-growing bamboo species with high economic, social and cultural value. The method of transplanting moso bamboo seedlings for afforestation has become a more economical and effective method. The effect of light on the growth of plant seedlings is mainly reflected in the regulation of different light quality on the growth and development of seedlings, including light morphogenesis, photosynthesis and secondary metabolites. Therefore, studying the effects of specific wavelength light on the physiology and proteome of moso bamboo seedlings will play an important role in growing seedlings and seed cultivation of moso bamboo. Here, moso bamboo seeds were germinated in the dark and then were transferred to the blue and red-light conditions. After 14 days, we observed the effects of different light treatments on the growth and development of seedlings, and then compared and analyzed their proteome.

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