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Next Generation Sequencing Facilitates Quantitative Analysis of overexpression ydeH and ydeH (E208,209A) Transcriptomes in Mycobacterium smegmatis

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of overexpression ydeH and ydeH (E208,209A) Transcriptomes in Mycobacterium smegmatis

PROVIDER: PRJNA414326 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR6174450_1.fastq.gz	Fastqsanger.gz
	SRR6174450_2.fastq.gz	Fastqsanger.gz
	SRR6174451_1.fastq.gz	Fastqsanger.gz
	SRR6174451_2.fastq.gz	Fastqsanger.gz
	SRR6174452_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing Facilitates Quantitative Analysis of Mycobacterium smegmatis C2 155 Transcriptomes treated with overexpression strains MS_Vec and MS_Rv2872

Project description:To gain molecular insight into the role of Rv2872 in increased vancomycin resistance, change of the intracellular amino acids content and cell wall structure, the transcriptome of Rv2872 overexpression M. smegmatis was assessed through RNA-Seq analysis. The transcriptome data showed that the transcription of four genes involved in peptidoglycan synthesis was significantly lower in the MS_Rv2872 overexpression strain . The decreased vancomycin targets might underlie the observed vancomycin resistance. Consistent with altered intracellular amino acids levels in the recombinant M. smegmatis MS_Rv2872, the transcription level of relevant genes was also altered. This is also seen in the altered fatty acids levels. The data showed that 38 fatty acid metabolism genes were down regulated, and 15 were up regulated in the recombinant M.smegmatis MS_Rv2872.

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Next Generation Sequencing Facilitates Quantitative Analysis of overexpression ydeH and ydeH (E208,209A) Transcriptomes in Mycobacterium smegmatis

Project description:Compare the gene expression difference between these two strains by RNA sequencing in Mycobacterium smegmatis

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Next Generation Sequencing Facilitates Quantitative Analysis of Mycobacterium smegmatis C2 155 Transcriptomes treated with overexpression strains MS_Vec and MS_Rv2872

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Project description:Compare the gene expression difference between ltmA knockout strains and wild-type strains by RNA sequencing in Mycobacterium smegmatis. The goal of this study is that detection LtmA acts as a transcriptional factor and regulates the target genes expression.

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