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Transcriptional change of HTR8/Svneo cells over expressed with PLAC8-EGFP

ABSTRACT: Transcriptional change of HTR8/Svneo cells over expressed with PLAC8-EGFP

PROVIDER: PRJNA415391 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6202831_1.fastq.gz	Fastqsanger.gz
	SRR6202831_2.fastq.gz	Fastqsanger.gz
	SRR6202832_1.fastq.gz	Fastqsanger.gz
	SRR6202832_2.fastq.gz	Fastqsanger.gz
	SRR6202833_1.fastq.gz	Fastqsanger.gz

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Transcriptional change of HTR8/Svneo cells over expressed with PLAC8-EGFP

Project description:The goal of this this project was to study the transcriptional change when PLAC8-EGFP was over expressed in the trophoblast cell line, HTR8/Svneo.

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Comparative proteome of the BeWo and HTR8/SVneo trophoblastic cell lines

Project description:Here, we have undertaken comparative label-free quantitative (LFQ) proteomic profiling of the proteins expressed in BeWo and HTR8/SVneo cell lines using a mass spectrometry approach. We have identified 1557 proteins in total. 915 proteins were expressed in both cell lines including various transcription factors, chaperones and transport proteins. A further 338 and 304 proteins were uniquely expressed in BeWo and HTR8/SVneo cells respectively. Our bioinformatics analysis reflects the known epithelial and mesenchymal phenotypes of these cell models with principal differences in GO observed in ‘Cell junction’, ‘Catenin complex’ as well as ‘Cell adhesion’ and ‘Cell differentiation’. Our novel comparative proteomic analysis of these trophoblastic cell lines will set the stage for the use of trophoblastic cell lines for the study of pregnancy disorders and further research focusing on placental function.

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Effect of depletion of TET3 on gene expression of HTR8-SVneo trophoblast cells.

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Project description:The goal of this study is to compare mRNAs expressed by EGF treated HTR-8/SVneo cells to iRNAs expressed in untreated control HTR-8/SVneo cells to identify various genes which play a role during EGF-mediated HTR-8/SVneo cell invasion

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SIRT1 binding proteins in human first trimester villi and HTR8/SVneo cells

Project description:In this study, we investigated the potential downstream target proteins in trophoblasts that were regulated by SIRT1 through modulation of acetylation. To this end, the IP products of SIRT1 in the lysate of human first-trimester villous tissue and HTR8/SVneo cells were subjected to proteomics analyses by LC-MS/MS.

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METTL3 regulates chromatin of human trophoblasts

Project description:To investigate the role of METTL3-mediated chromatin dynamics, we performed ChIP-seq for METTL3, ARID1A and H3K27ac,and ATAC-seq in high glucose treated control or METTL3 knockdown HTR8/SVneo cells.

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Effect of depletion of TET3 on gene expression of HTR8-SVneo trophoblast cells.

Project description:Effect of depletion of TET3 on gene expression of HTR8-SVneo trophoblast cells.

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N6-methyladenosine (m6A) profiling and translatome of human trophoblast

Project description:To investigate the role of METTL3-mediated m6A modification and mRNA translation, we performed m6A-sequencing and Ribosome-nascent chain (RNC)-RNA sequencing in high glucose treated control or METTL3 knockdown HTR8/SVneo cells.

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