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Syndecan-1 controls lung tumorigenesis by regulating microRNAs packaged in exosomes [A549]

ABSTRACT: Syndecan-1 controls lung tumorigenesis by regulating microRNAs packaged in exosomes [A549]

PROVIDER: PRJNA416109 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6230320.fastq.gz	Fastqsanger.gz
	SRR6230321.fastq.gz	Fastqsanger.gz
	SRR6230322.fastq.gz	Fastqsanger.gz
	SRR6230323.fastq.gz	Fastqsanger.gz
	SRR6230324.fastq.gz	Fastqsanger.gz

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Syndecan-1 controls lung tumorigenesis by regulating microRNAs packaged in exosomes [A549]

Project description:We evaluated the effect of syndecan-1 in various cell-based and animal models of lung cancer and found that lung tumorigenesis was promoted when cells lose syndecan-1 expression. We also demonstrate that syndecan-1 (or lack thereof) alters the microRNA (miRNA) cargo carried within exosomes exported from lung cancer cells. Analysis of the changes in miRNA expression identifies a distinct shift toward augmented pro-cancerous signaling, which are consistent with the changes found in lung adenocarcinoma. In all, our work identifies syndecan-1 as an important factor on lung cancer cells that control its ability to shape the lung tumor microenvironment through alterations in miRNA packaging within exosomes.

2017-10-28 | GSE106277 | GEO

Syndecan-1 controls lung tumorigenesis by regulating microRNAs packaged in exosomes [EKVX]

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Syndecan-1 controls lung tumorigenesis by regulating microRNAs packaged in exosomes

Project description:Syndecan-1 controls lung tumorigenesis by regulating microRNAs packaged in exosomes

| PRJNA416251 | ENA

Syndecan-1 controls lung tumorigenesis by regulating microRNAs packaged in exosomes [EKVX]

Project description:Syndecan-1 controls lung tumorigenesis by regulating microRNAs packaged in exosomes [EKVX]

| PRJNA416253 | ENA

Syndecan-1 controls lung tumorigenesis by regulating microRNAs packaged in exosomes

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-10-30 | GSE106304 | GEO

Syndecan-1 Controls Lung Tumorigenesis by Regulating miRNAs Packaged in Exosomes.

Project description:Syndecan-1 is a transmembrane proteoglycan expressed prominently by lung epithelium and has pleiotropic functions such as regulating cell migration, proliferation, and survival. Loss of syndecan-1 expression by lung cancer cells is associated with higher-grade cancers and worse clinical prognosis. We evaluated the effects of syndecan-1 in various cell-based and animal models of lung cancer and found that lung tumorigenesis was moderated by syndecan-1. We also demonstrate that syndecan-1 (or lack thereof) alters the miRNA cargo carried within exosomes exported from lung cancer cells. Analysis of the changes in miRNA expression identified a distinct shift toward augmented procancer signaling consistent with the changes found in lung adenocarcinoma. Collectively, our work identifies syndecan-1 as an important factor in lung cancer cells that shapes the tumor microenvironment through alterations in miRNA packaging within exosomes.

| S-EPMC5963476 | biostudies-literature

miRNA expression of exosomes derived from lung cancer cells A549 and cisplatin (DDP) resistant lung cancer cells A549/DDP

Project description:To investigate the difference of miRNA expression in exosomes derived from A549 cells and its DDP-resistant cell strain A549/DDP, we have employed miRNA microarray expression to discover the difference expression of miRNAs in exosomes derived from A549 and A549/DDP. We conducted RT-qPCR to examine the expression levels of top differential expressed miRNAs, namely, miR-197-5p, miR-4443, miR-642a-3p, miR-27b-3p and miR-100-5p, confirming low variability between two methods. The A549/DDP was established from A549 in our laboratory, by exposing A549 to gradually increasing DDP concentrations, until the final concentration at 1Î¼g/ml. To avoid the influence of drug to the A549/DDP cells, they were cultured in a drug-free medium for at least two weeks before gene expression analysis. After being incubated for 48-72h, the culture medium of cells was harvested. Exosomes were isolated by ultracentrifugation. And miRNA expression of exosomes derived from A549 and A549/DDP was then analzyed.

2016-08-16 | E-GEOD-85604 | biostudies-arrayexpress

Project description:Breast milk is a complex liquid that enriched in immunological components and affect the development of the infant immune system. Exosomes, the membranous vesicles of endocytic origin, are ubiquitously in various body fluids which can mediate intercellular communication. MicroRNAs (miRNAs), a well-defined group of non-coding small RNAs, in human breast milk are packaged inside exosomes. Here, we present the identification of miRNAs in human breast milk exosomes using deep sequencing technology. We found that the immune-related miRNAs are enriched in breast milk exosomes, and are resistant to the general harsh conditions. Four small RNA libraries in human breast milk exosomes from four healthy women (30 +/- 0.9 years old, primiparity) when the infant were aged at 60 days were sequenced.

2011-12-21 | E-GEOD-32253 | biostudies-arrayexpress

2011-12-21 | GSE32253 | GEO

miRNA expression of exosomes derived from lung cancer cells A549 and cisplatin (DDP) resistant lung cancer cells A549/DDP

2016-08-16 | GSE85604 | GEO