Plants
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA416208 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Membraneless biomolecular condensates formed through liquid-liquid phase separation (LLPS) are known to play key roles in plant growth and stress responses. How plant viruses utilize LLPS to escape host immunity remains largely unexplored. Here, we present evidence demonstrating that the P6 protein encoded by southern rice black-streaked dwarf virus (SRBSDV) undergoes LLPS. P6 interacts with OsTSN1 and triggers the assembly of P6-OsTSN1-containing droplets that co-localize with stress granules (SGs). Within these P6-OsTSN1-containing droplets, P6 enhances the nuclease activity of OsTSN1 via promoting its multimerization to degrade transcripts with G-A-rich motifs of two transcription factors (TFs), OsNAC15 and OsLHY. These TFs regulate the transcription of OsJAZ6, OsJAZ12, and OsATG8C, involved in jasmonic acid (JA)- and autophagy-associated defense pathways in plants. Additionally, the degradation of OsNAC15 and OsLHY transcripts in the P6-OsTSN1-containing droplets in SGs weakens JA- and autophagy-mediated defenses in rice, facilitating SRBSDV infection. Interestingly, similar to SRBSDV P6, intrinsically disordered region (IDR)-containing RNA silencing suppressors encoded by other rice viruses such as rice black-streaked dwarf virus and rice stripe virus, also interact with OsTSN1, promoting the degradation of OsNAC15 and OsLHY transcripts to enhance viral infection. Our findings indicate that OsTSN1 acts as a central positive regulator of virus infection in rice, convergently co-opted by viruses. These insights help us to better understand the roles of LLPS and OsTSN1 in virus infection in rice.
2025-07-07 | GSE283860 | GEO
Project description:Membraneless biomolecular condensates formed through liquid-liquid phase separation (LLPS) are known to play key roles in plant growth and stress responses. How plant viruses utilize LLPS to escape host immunity remains largely unexplored. Here, we present evidence demonstrating that the P6 protein encoded by southern rice black-streaked dwarf virus (SRBSDV) undergoes LLPS. P6 interacts with OsTSN1 and triggers the assembly of P6-OsTSN1-containing droplets that co-localize with stress granules (SGs). Within these P6-OsTSN1-containing droplets, P6 enhances the nuclease activity of OsTSN1 via promoting its multimerization to degrade transcripts with G-A-rich motifs of two transcription factors (TFs), OsNAC15 and OsLHY. These TFs regulate the transcription of OsJAZ6, OsJAZ12, and OsATG8C, involved in jasmonic acid (JA)- and autophagy-associated defense pathways in plants. Additionally, the degradation of OsNAC15 and OsLHY transcripts in the P6-OsTSN1-containing droplets in SGs weakens JA- and autophagy-mediated defenses in rice, facilitating SRBSDV infection. Interestingly, similar to SRBSDV P6, intrinsically disordered region (IDR)-containing RNA silencing suppressors encoded by other rice viruses such as rice black-streaked dwarf virus and rice stripe virus, also interact with OsTSN1, promoting the degradation of OsNAC15 and OsLHY transcripts to enhance viral infection. Our findings indicate that OsTSN1 acts as a central positive regulator of virus infection in rice, convergently co-opted by viruses. These insights help us to better understand the roles of LLPS and OsTSN1 in virus infection in rice.
2025-07-07 | GSE283861 | GEO
Project description:Human PDCD4 wild-type (wt) promoter fragments were amplified from U2OS genomic DNA and X-box deletion mutants (mut) were generated using the QuikChange site-directed mutagenesis kit (Agilent Technologies). DNA probes for affinity purification with the same sequences were obtained by PCR using a biotinylated primer for labeling the 3' end (Thermo Fisher Scientific). DNA affinity purification with nuclear extracts from Nutlin-3a treated U2OS cells.
2024-08-08 | PXD020932 | Pride
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Project description:Mutualistic symbionts are increasingly recognized as hidden players in plant-insect interactions. Understanding how symbionts affect plant responses to herbivory provides important insight into the fascinating complexity of terrestrial ecological networks and how we could specifically target symbionts to achieve sustainable pest management. Although the role of herbivore-associated symbionts is gaining increased attention in ecology of plant-insect interactions, how symbionts of members of higher trophic levels affect these interactions is poorly understood. The natural enemies of insect herbivores such as parasitic wasps that are used in biological pest control are associated with mutualistic symbionts (polydnaviruses) that they inject into the herbivore along with eggs in an event of parasitisation. We showed previously that polydnavirus-genes are expressed in the salivary glands of parasitized caterpillars suggesting that third-trophic level symbionts may alter the herbivore’s salivary composition and consequently, affect the way the herbivore interacts with the plant. By using a novel multidisciplinary approach that integrates molecular, chemical, behavioral and field ecology, the proposed project aims to manipulate the phenotype of caterpillars to study the effect of symbiotic viruses of third-trophic-level insects at the plant-herbivore interface. We will address this issue at different levels of biological organization, from plant gene expression to plant-mediated ecological consequences. The project will thereby provide novel fundamental insights into the hidden role played by parasitic wasp symbionts in plant responses to herbivory and these insights will generate important knowledge for pest management programs that focus on polydnaviruses as biological control agents.
2025-08-25 | PXD060225 | Pride
Project description:Volatile organic compounds (VOCs) may play a role in systemic acquired resistance (SAR), a salicylic acid (SA)-associated, broad-spectrum immune response in systemic, healthy tissues of locally infected plants. Four-to-five-week-old plants were exposed for three days to a mixture of volatile pinenes to investigate genome-wide transcriptional responses relative to hexane-treated control plants.
2017-06-01 | E-MTAB-5475 | biostudies-arrayexpress