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Danio rerio

ABSTRACT: Nuclear Receptor Disruption Alters Triphenyl Phosphate-Induced Cardiotoxicity in Zebrafish Embryos

PROVIDER: PRJNA416833 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6251100.fastq.gz	Fastqsanger.gz
	SRR6251110.fastq.gz	Fastqsanger.gz
	SRR6251101.fastq.gz	Fastqsanger.gz
	SRR6251111.fastq.gz	Fastqsanger.gz
	SRR6251102.fastq.gz	Fastqsanger.gz

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Role of the Exon Junction Complex in Danio rerio

Project description:The exon junction complex (EJC) is composed of three core proteins Rbm8a, Magoh and Eif4a3 and is thought to play a role in several post-transcriptional processes. In this study we focus on understanding the role of EJC in zebrafish development. We identified transcriptome-wide binding sites of EJC in zebrafish via RNA:protein immunoprecipitation followed by deep sequencing (RIP-Seq). We find that, as in human cells, zebrafish EJC is deposited about 24 nts upstream of exon-exon junctions. We also identify transcripts regulated by Rbm8a and Magoh in zebrafish embryos using whole embryo RNA-seq from rbm8a mutant, magoh mutant and wild-type sibling embryos. This study shows that nonsense mediated mRNA decay is dysregulated in zebrafish EJC mutants.

2019-07-30 | GSE135019 | GEO

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