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TGFb treated EpRAS cells undergoing hysteretic vs non-hysteretic EMT gene expression analysis.

ABSTRACT: TGFb treated EpRAS cells undergoing hysteretic vs non-hysteretic EMT gene expression analysis.

PROVIDER: PRJNA417150 | ENA |

REPOSITORIES: ENA

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TGFb treated EpRAS cells undergoing hysteretic vs non-hysteretic EMT gene expression analysis.

Project description:EpRAS cells undergo EMT transition through hysteresis showing bistability of the system. The hysteresis transition is controlled by the negative feedback loop between miR-200s and Zeb1. Disruption of the feedback loop through the deletion of Z-box2 in the miR-200s cluster-2 promoter region changes the transition to unimodal without hysteresis. To determine the consequence of cells undergoing through hysteresis or unimodal transitions of EMT, herein we determined the transcriptomic profiles of EpRAS wild type and EpRAS-delZbox2 mutant cells, (designated as EpRAS-C2-2). To induce EMT, the cells were treated with 100pM TGFbeta for 3 days. Mock PBS treatment is used as control.

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WDR5 regulates EMT and metastasis in breast cancer by activating TGFB pathway [RNA-seq PDX]

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miRNA expression profiling in PANC-1 cells treated with TGFb and additional PDAC lines

Project description:miRNAs are known to be involved in PDAC tumourigenesis. However, not many have been investigated in the context of TGFb-induced EMT. To identify novel miRNAs involved in this response, we performed a miRNA profiling study in PANC-1 cells treated with TGFb and additional PDAC lines with epithelial or mesenchymal characteristics.

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