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Gene expression profiling of NSCLC cell lines upon TGFb-induced epithelial-mesenchymal transition (EMT)

ABSTRACT: Epithelial-mesenchymal transition has been implicated in tumor metastasis, cancer drug resistance and cancer stem cell features. In this study, we examined gene expression profiles of three non-small cell lung cancer cell lines, before and after experimentally induced EMT. We modeled epitheial-mesenchyml transition by culturing A549, HCC827 and NCI-H358 cells in the presence of TGFb for three weeks, and compared gene expression profiles of the paretnal cells and cells after EMT.

ORGANISM(S): Homo sapiens

SUBMITTER: Yuting Sun

PROVIDER: E-GEOD-49644 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Metabolic and transcriptional profiling reveals pyruvate dehydrogenase kinase 4 as a mediator of epithelial-mesenchymal transition and drug resistance in tumor cells.

Sun Yuting Y Daemen Anneleen A Hatzivassiliou Georgia G Arnott David D Wilson Catherine C Zhuang Guanglei G Gao Min M Liu Peter P Boudreau Aaron A Johnson Leisa L Settleman Jeff J

Cancer & metabolism 20141103 1

<h4>Background</h4>Accumulating preclinical and clinical evidence implicates epithelial-mesenchymal transition (EMT) in acquired resistance to anticancer drugs; however, mechanisms by which the mesenchymal state determines drug resistance remain unknown.<h4>Results</h4>To explore a potential role for altered cellular metabolism in EMT and associated drug resistance, we analyzed the metabolome and transcriptome of three lung cancer cell lines that were rendered drug resistant following experiment ...[more]

PMID: 25379179

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