Project description:Hyphopichia burtonii has much stronger osmotolerance than model Saccharomyces cerevisiae and other yeast. The transcriptomic changes by RNA-seq analysis in several hyperosmotic stresses and YPD condition as control were investigated, expecting changes in the expression patterns of genes that do not exist or change in other yeast.
Project description:Transcriptome data of Hyphopichia burtonii and Hyphopichia pseudoburtonii when exposed to high osmosterss (1M NaCl) condtion at several time points.
Project description:Hyphopichia pseudoburtonii, is considered as promising biological control agents due to its large spectrum of antifungal activity. H.pseudoburtonii strain Y963 isolated from grape must in Stellenbosch, possesses significant antifungal activity against B. cinerea, the causal agent of grey mould, a significant disease affecting grapes world-wide. Several modes of action, like competition for nutrients and space, production of extracellular lytic enzymes and volatile organic compounds, have been demonstrated for the biological control activity exhibited by H. pseudoburtonii through which it inhibits B. cinerea. To reveal the underlying antifungal genetic mechanisms, this study examined the changes in gene expression of H. pseudoburtonii when exposed to B. cinerea over 24, 48, and 120 h using RNA sequencing. The results show that H. pseudoburtonii undergoes a responsive reaction upon encountering B. cinerea, leading to increased production of nucleotides and proteins. Subsequently, a period followed during which various forms of stress were induced. This led to increased signaling and cell communication, as well as the activation of genes responsible for pseudohyphal growth. In later stages, vigorous growth and competition for nutrients became apparent. Up-regulation of genes associated with transmembrane functions, iron homeostasis, zinc homeostasis, hydrolases, and glycosyl hydrolases was observed at 120 h. This research offers new insights into H.pseudoburtonii transcriptomic response to B.cinerea and illuminates the adaptive strategies and molecular mechanisms behind its antifungal activity.
Project description:Azole resistance was induced in vitro by growth of a susceptible C. parapsilosis isolate in the presence of voriconazole. Whole genome microarrays were used to compare the transcriptional response of the voriconizole-resistant and susceptible isolates.
Project description:Azole resistance was induced in vitro by growth of a susceptible C. parapsilosis isolate in the presence of fluconazole. Whole genome microarrays were used to compare the transcriptional response of the fluconazole-resistant and susceptible isolates.