Metagenomic assembled genomes from three ice covered boreal lakes
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PROVIDER: PRJNA417934 | ENA |
REPOSITORIES: ENA
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Project description:Metagenomic assembled genomes from three ice covered boreal lakes
| PRJNA417044 | ENA
Project description:With changes in ice cover duration, nutrient loading, and anoxia risk, it is important to understand the mechanisms that control nitrogen cycling and oxygen depletion in lakes through winter. Current understanding is largely limited to description of changes in chemistry, with few measurements of the processes driving winter changes, how they differ across lakes, and how they are impacted by under-ice conditions. Nitrification is a process which consumes oxygen and ammonium (NH4+), and supplies nitrate (NO3-). To date, nitrification has been measured under ice cover in only two lakes globally. Here, we used 15NH4+ enrichment to measure rates of pelagic nitrification in thirteen water bodies in two ecozones. Our work demonstrates ecologically important rates of nitrification can occur despite low water temperatures, impacting NH4+, NO3- and, most importantly, oxygen concentrations. However, high rates are not the norm. When, where and why is nitrification important in winter? We found that nitrification rates were highest in a eutrophic lake chain downstream of a wastewater treatment effluent (mean: 226.5 ?g N L-1 d-1), and in a semi-saline prairie lake (110.0 ?g N L-1 d-1). In the boreal shield, a eutrophic lake had nitrification rates exceeding those of an oligotrophic lake by 6-fold. Supplementing our results with literature data we found NH4+ concentrations were the strongest predictor of nitrification rates across lentic ecosystems in winter. Higher nitrification rates were associated with higher concentrations of NH4+, NO3- and nitrous oxide (N2O). While more work is required to understand the switch between high and low nitrification rates and strengthen our understanding of winter nitrogen cycling, this work demonstrates that high nitrification rates can occur in winter.
| S-EPMC6837456 | biostudies-literature
Project description:Dark carbon fixation in stratified boreal lakes water column
| PRJEB76863 | ENA
Project description:Ice metagenome of glaciers and crater lakes from the Iztaccihuatl volcanic complex
| PRJNA476152 | ENA
Project description:Verrucomicrobia metagenomes assembled genomes from seasonally ice-covered lakes in Quebec, Canada
| PRJNA480789 | ENA
Project description:Boreal toads (Anaxyrus boreas boreas) of the Southern Rocky Mountain population are declining due to the introduction of the chytrid fungus Batrachochytrium dendrobatidis (Bd). Boreal toads in Colorado are generally susceptible to Bd infection, but some Bd-tolerant populations persist in parts of the Southern Rocky Mountain and broader Eastern boreal toad population. We conducted a Bd challenge with lab-reared sibling toads from Bd-susceptible Colorado and purportedly Bd-tolerant Utah populations and report on transcriptomic responses to Bd during late infection in skin and liver tissue. Fewer immune genes were expressed in response to Bd in Colorado toads, but with greater upregulation compared to Utah toads, indicating a dysregulated immune response. Signatures of Bd-tolerance in Utah toads included more moderate upregulation in immune gene expression and a significantly enriched suite of gene functions related to innate and adaptive immune responses. Our transcriptomic results support the notion that Utah toads are tolerant to Bd, rather than resistant, carrying Bd loads similar to Colorado yet having a unique transcriptomic profile and presenting minimal clinical signs of chytridiomycosis. We conclude that closely related populations have divergent transcriptomic responses to Bd with a dysregulated immune response in Bd-susceptible toads.
2023-10-04 | GSE244617 | GEO
Project description:Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition
| PRJEB18567 | ENA
Project description:In this study we used metaproteomics to discern the metabolism and physiology of the microorganisms occurring in the phototrophic mats of four soda lakes in the interior of British Columbia, Canada. Binned and assembled metagenomes were used as the database for protein identification.
2019-07-29 | PXD011230 | Pride