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Global transcriptome of Arabidopsis thaliana and Solanum lycopersicum infected by S. sclerotiorum

ABSTRACT: Global transcriptome of Arabidopsis thaliana and Solanum lycopersicum infected by S. sclerotiorum

OTHER RELATED OMICS DATASETS IN: GSE106811

PROVIDER: PRJNA418121 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6283150.fastq.gz	Fastqsanger.gz
	SRR6283151.fastq.gz	Fastqsanger.gz
	SRR6283152.fastq.gz	Fastqsanger.gz
	SRR6283143.fastq.gz	Fastqsanger.gz
	SRR6283153.fastq.gz	Fastqsanger.gz

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Global transcriptome of Arabidopsis thaliana and Solanum lycopersicum infected by S. sclerotiorum

Project description:Plant pathogens with a broad host range are able to infect plant lineages that diverged over 100 million years ago. They exert similar and recurring constraints on the evolution of unrelated plant populations. Plants generally respond with quantitative disease resistance (QDR), a form of immunity relying on complex genetic determinants. In most cases, the molecular determinants of QDR and how they evolve is unknown. Here we identify in Arabidopsis thaliana a gene mediating QDR against Sclerotinia sclerotiorum, agent of the white mold disease, and provide evidence of its convergent evolution in multiple plant species. Using genome wide association mapping in A. thaliana, we associated the gene encoding the POQR prolyl-oligopeptidase with QDR against S. sclerotiorum. Loss of this gene compromised QDR against S. sclerotiorum but not against a bacterial pathogen. Natural diversity analysis associated POQR sequence with QDR. Remarkably, the same amino acid changes occurred after independent duplications of POQR in ancestors of multiple plant species, including A. thaliana and tomato. Genome-scale expression analyses revealed that parallel divergence in gene expression upon S. sclerotiorum infection is a frequent pattern in genes, such as POQR, that duplicated both in A. thaliana and tomato. Our study identifies a previously uncharacterized gene mediating QDR against S. sclerotiorum. It shows that some QDR determinants are conserved in distantly related plants and have emerged through the repeated use of similar genetic polymorphisms at different evolutionary time scales.

2017-12-12 | GSE106811 | GEO

microRNA-target RNA pairs revealed by Parallel analysis of RNA ends in Solanum lycopersicum

Project description:Solanum lycopersicum RNA degradome sequencing

2012-08-29 | GSE22236 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Solanum lycopersicum bud and flower Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Solanum lycopersicum bud and flower Transcriptomes

2021-02-27 | GSE164382 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Solanum lycopersicum Axillary bud Transcriptomes

Project description:RNA sequencing in tomato for detect mRNA expression of Solanum lycopersicum Axillary bud.The two cultivars (monomaker, raceme) at Axillary bud for transcriptome sequencing

2021-12-31 | GSE164164 | GEO

The Solanum lycopersicum WRKY3 Transcription Factor Regulates Salt Stress Tolerance In Tomato

Project description:The tomato SlWRKY3 transcription factor was overexpressed in cultivated tomato (Solanum lycopersicum)and transgenic plants transcriptome was compared to that of wild-type plants.

2016-03-15 | GSE74504 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Solanum lycopersicum Flower Transcriptomes

Project description:RNA sequencing in tomato for detect mRNA expression of Solanum lycopersicum flower.The two cultivars (monomaker, raceme) had three different flowering stages (budlet, Flower bud, Full bloom) for transcriptome sequencing

2021-08-11 | GSE163914 | GEO

Spectral library of Solanum lycopersicum for SWATH-MS

Project description:A spectral library was built for Solanum lycopersicum. The spectral library allows reproducible quantification for thousands of peptides per SWATH-MS analysis. Proteins from Solanum lycopersicum pericarp were digested with trypsin using in-gel digestion and the peptides were fractionated by high-pH reverse phase chromatography. HRM peptides were spiked into the peptides mixture and each fraction was injected on a Sciex TripleTOF 6600 mass spectrometer fitted with microflow set-up. The resulting .wiff files were analysed using MaxQuant and Spectronaut.

2017-05-11 | MSV000081074 | MassIVE

Solanum lycopersicum

Project description:Solanum lycopersicum

| PRJNA252089 | ENA

microRNA-target RNA pairs revealed by Parallel analysis of RNA ends in Solanum lycopersicum

Project description:Solanum lycopersicum RNA degradome sequencing Isolated polyadenylated RNA from total RNA extracts of Solanum lycopersicum, were ligated to 5'-adapter that includes an MmeI recognition site. The ligated products were purified again, reverse transcribed and cleaved with MmeI. The 5' fragments were purified from gel and ligated to a 3'- dsDNA adapter and PCR amplified. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

2012-08-29 | E-GEOD-22236 | biostudies-arrayexpress

Solanum lycopersicum Transcriptome or Gene expression

Project description:Lines nearly isogenic for fw3.2 in the cultivated background Solanum lycopersicum c.v. Yellow Stuffer were grown in the greenhouse in a completely randomized design. fw3.2 (ys) and fw3.2 (wt) are NILs carrying cultivated and wild alleles for fw3.2 locus. Young flower buds were harvested. For each sample, three replicates were used. RNA was extracted using Trizol and Stand-specific libraries were prepared from total RNA and sequences of 51 bp were generated on an Illumina HiSeq2000.

2013-07-27 | E-MTAB-4793 | biostudies-arrayexpress

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