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Expression data from Huh7.5.1 cells transfected with siNDRG1 or siNT

ABSTRACT: Expression data from Huh7.5.1 cells transfected with siNDRG1 or siNT

PROVIDER: PRJNA418726 | ENA |

REPOSITORIES: ENA

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Expression data from Huh7.5.1 cells transfected with siNDRG1 or siNT

Project description:Host cells harbor various intrinsic mechanisms to restrict viral infections as a first line of antiviral defense. Viruses have evolved various countermeasures against these antiviral mechanisms. Here we show that N-Myc Downstream-Reguated Gene 1 (NDRG1) limits productive HCV infection by inhibiting viral assembly. Interestingly, HCV infection down-regulates NDRG1 protein and mRNA expression. Loss of NDRG1 increases the size and number of lipid droplets, which are the sites of HCV assembly. HCV suppresses NDRG1 expression by up-regulating MYC, which directly inhibits the transcription of NDRG1. Up-regulation of MYC also leads to reduced expression of NDRG1-specific kinase SGK1, resulting in markedly diminished phosphorylation of NDRG1. Knockdown of MYC during HCV infection rescues NDRG1 expression and phosphorylation, suggesting that MYC regulates NDRG1 at both transcriptional and post-translational levels. Overall, our results suggest that NDRG1 restricts HCV assembly by limiting lipid droplet formation. HCV counteracts this intrinsic antiviral mechanism by down-regulating NDRG1 via a MYC-dependent mechanism.

2017-11-17 | GSE106988 | GEO

RNA-sequencing of siNT and siRAD52 transfected HeLa cells

Project description:Gene expression profiling by RNA sequencing of control (siNT) and RAD52-depleted (siRAD52) HeLa cells.

2024-07-19 | GSE272608 | GEO

Gene expression analysis of Neoechinulin B (NeoB)-treated Huh7.5.1 cells

Project description:Huh-7.5.1 cells were treated with 0.2% DMSO, 20 microM NeoB for 24 h. Treatment with 0.2% DMSO for 24h was prepared as non-treated Huh7.5.1 cells. Huh7.5.1 cells were kindly provided by Prof. Francis Chisari at The Scripps Research Institute.

2015-08-31 | GSE63026 | GEO

Gene expression analysis of Neoechinulin B (NeoB)-treated Huh7.5.1 cells

2015-08-31 | E-GEOD-63026 | biostudies-arrayexpress

Expression Data from siRae1 and siNT infected with VSV or mock infected

Project description:To test the effect of silencing Rae1 on expression on RNA polymerase II transcripts, host mRNAs were analysed by cDNA microarrays. We hypothesized that if silencing Rae1 expression increases cellular resistance to inhibition of transcription in VSV infected cells, mRNA characteristic of host antiviral response would be increased than compared to cells transfected with control siRNA. HeLa cells transfected with siRNA targeted against Rae1 (siRae1) or against non-targeting siRNA (siNT). The sequence of nontargeting (NT) siRNA is scrambled and does not match any sequence on the human genome. Transfected cells were either infected with VSV or mock infected. Six hours later RNA was extracted from the cells. Two separate experiments of such were analyzed by microarrays

2012-06-28 | E-GEOD-38866 | biostudies-arrayexpress

Cellular microRNAs in Huh7.5.1 cells

Project description:Cellular microRNAs in Huh7.5.1 cells

| PRJNA563554 | ENA

Phosphoproteome of Hepatitis C Virus infected Huh7.5.1 cells

Project description:This project enriched and identified phosphoproteins in human hepatocarcinoma 7.5.1 cell line (Huh7.5.1) upon Hepatitis C virus (HCV) infection.

2016-07-08 | PXD000988 | Pride

JFH1-huh7.5.1

Project description:Genome wide DNA methylation analysis usig two individual huh7.5.1 after HCV-JFH1 infection

2014-01-03 | E-MTAB-1473 | biostudies-arrayexpress

Expression data from LEF siRNA transfected Jurkat cells

Project description:Expression data from LEF siRNA transfected Jurkat cells

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Endoribonuclease activity in hepatitis C virus-infected Huh7.5.1 cells

Project description:We used 2', 3'-cyclic phosphate cDNA synthesis and Illumina sequencing to identify and endoribonuclease cleavage sites in host and viral RNAs during HCV infection of Huh7.5.1 cells

2022-08-06 | GSE72144 | GEO

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