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Pseudotaxus chienii

ABSTRACT: Pseudotaxus chienii Raw sequence reads

PROVIDER: PRJNA419098 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	SRR6447674.fastq.gz	Fastqsanger.gz
	SRR6447674_1.fastq.gz	Fastqsanger.gz
	SRR6447674_2.fastq.gz	Fastqsanger.gz

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The genome of Pseudotaxus chienii, a relict conifer endemic to China, provides insights into the origin and evolution of taxane biosynthesis

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Project description:Purpose: The goal of this study is to compare endothelial small RNA transcriptome to identify the target of OASL under basal or stimulated conditions by utilizing miRNA-seq. Methods: Endothelial miRNA profilies of siCTL or siOASL transfected HUVECs were generated by illumina sequencing method, in duplicate. After sequencing, the raw sequence reads are filtered based on quality. The adapter sequences are also trimmed off the raw sequence reads. rRNA removed reads are sequentially aligned to reference genome (GRCh38) and miRNA prediction is performed by miRDeep2. Results: We identified known miRNA in species (miRDeep2) in the HUVECs transfected with siCTL or siOASL. The expression profile of mature miRNA is used to analyze differentially expressed miRNA(DE miRNA). Conclusions: Our study represents the first analysis of endothelial miRNA profiles affected by OASL knockdown with biologic replicates.

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