Project description:Multi-modal single-cell assays provide high-resolution snapshots of complex cell populations but are mostly limited to transcriptome plus an additional modality. Here, we describe Expanded CRISPR-compatible Cellular Indexing of Transcriptomes and Epitopes by sequencing (ECCITE-seq) for the high-throughput characterization of at least five modalities of information from each single cell. We demonstrate application of ECCITE-seq to multimodal CRISPR screens with robust direct sgRNA capture and to clonotype-aware multimodal phenotyping of cancer samples.
2019-03-01 | GSE126310 | GEO
Project description:DNA sequencing data of Salvia chienii
Project description:To allow the characterization of the populations of small RNAs in the testes of mutant animal, small-RNA fraction of total testis RNA was isolated and sequenced.
Project description:We used RNA sequencing to examine the transcriptomes of male and female heads from experimentally-evolved D. melanogaster populations after 117 generations of mating system manipulation in order to examine the pattern of evolution in sex-biased genes. Examined head transcriptomes of 3 monogamous populations and 3 polygamous populations, both males and females, for 12 total samples.
