Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional Regulation of Nitrogen and Nitrogen-Related Metabolism in Arabidopsis

ABSTRACT: Transcriptional Regulation of Nitrogen and Nitrogen-Related Metabolism in Arabidopsis

PROVIDER: PRJNA422118 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6369360.fastq.gz	Fastqsanger.gz
	SRR6369370.fastq.gz	Fastqsanger.gz
	SRR6369380.fastq.gz	Fastqsanger.gz
	SRR6369390.fastq.gz	Fastqsanger.gz
	SRR6369400.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 208

Similar Datasets

Elucidating transcriptional regulation of nitrogen metabolism with systems approaches

Project description:Elucidating transcriptional regulation of nitrogen metabolism with systems approaches

| PRJNA238009 | ENA

Regulation of nitrogen and carbon metabolism in Aspergillus nidulans.

Project description:In Aspergillus nidulans, nitrogen and carbon metabolism are under the control of wide-domain regulatory systems, including nitrogen metabolite repression, carbon catabolite repression. Transcriptomic analysis of the wild type strain grown under different combinations of carbon and nitrogen regimes was performed, to identify differentially regulated genes. Carbon metabolism predominates as the most important regulatory signal but for many genes, both carbon and nitrogen metabolisms coordinate regulation.

2019-05-15 | GSE115021 | GEO

Elucidating transcriptional regulation of nitrogen metabolism with systems approaches [RNA-Seq]

Project description:Elucidating transcriptional regulation of nitrogen metabolism with systems approaches [RNA-Seq]

| PRJNA238011 | ENA

Regulation of nitrogen and carbon metabolism in Aspergillus nidulans.

Project description:Regulation of nitrogen and carbon metabolism in Aspergillus nidulans.

| PRJNA473528 | ENA

Transcriptional Regulation of Nitrogen and Nitrogen-Related Metabolism in Arabidopsis

Project description:Purpose: Investigate transcriptional changes by genetic perturbation of specific transcription factors under sufficient and limiting nitrate conditions. Methods: 9 day old roots were harvested between 1-2pm (get hours after “sunrise”) and frozen in liquid N2. RNA-seq libraries were prepared following the BRAD-Seq DGE protocol (four biological replicates per genotype and treatment). Libraries were sequenced using the Ilummina HiSeq 3000 in SR50 mode. Two technical replicates libraries were created from each RNA sample; after assessing sufficient reproducibility, counts across technical replicates were pooled together. Reads were processed with fastx_trimmer for barcode removal (-f 9 -v -Q 33) and trimmed by quality with reaper (paremeters: -geom no-bc -dust-suffix-late 10/ACTG -dust-suffix 10/ACTG --noqc -nnn-check 1/1 -qqq-check 33/10 -clean-length 30 -tri 20 -polya 5 --bcq-late), then mapped to the reference genome of Arabidopsis thaliana (TAIR v10) using bowtie (-a --best --strata -n 1 -m 1 -p 4 --sam --tryhard) with subsequent conversion to BAM format using samtools and counts were obtained with HTSeq-count.

2018-07-30 | GSE107988 | GEO

Are small RNAs involved in the regulation of nitrogen metabolism in haloarchaea?

Project description:Small RNAs have been studied in detail in Bacteria and Eukarya domain, but in the case of Archaea domain the knowledge is scarce and the physiological function of the majority is still uncertain. To extend the knowledge of sRNAs in Archaea domain and its possible role in the regulation of the nitrogen assimilation metabolism in haloarchaea, Haloferax mediterranei has been used as a model microorganism. Bioinformatic approach has allowed to predict 295 putative sRNAs genes in the genome of H. mediterranei, 88 of which have been verified by means of RNA-seq. The secondary structure of putative sRNAs and its possible targets have been identified. Curiously, some of them present as possible targets genes related to the nitrogen assimilation, as glutamate dehydrogenase or regulatory nitrogen protein PII. Analysis of RNA-seq data has also revealed differences in the expression pattern of 16 sRNAs according to the nitrogen source. Consequently, RNomic and the bioinformatic approaches used in this work have allowed the identification of new sRNAs in Hfx. mediterranei, some of which show different expression pattern depending on the nitrogen source. It suggests that these sRNAs could be involved in the regulation of nitrogen assimilation, being able to constitute important gene regulatory network.

2018-02-20 | GSE108616 | GEO

Enhanced nitrogen removal via Yarrowia lipolytica-mediated nitrogen and related metabolism of Chlorella pyrenoidosa from wastewater

Project description:Enhanced nitrogen removal via Yarrowia lipolytica-mediated nitrogen and related metabolism of Chlorella pyrenoidosa from wastewater

| PRJNA964764 | ENA

Dual regulation of nitrogen metabolism by CodY and GlnR in Streptococcus mutans

Project description:CodY and GlnR are two major transcriptional regulators in nitrogen metabolism in Gram-positive bacteria. CodY regulates genes involved in the adaptive response to poor growth conditions, especially to nutrient limitation. GlnR controls nitrogen utilization according to the availability of nitrogen source. In this study we used microarray to investigate the regulatory roles that CodY and GlnR play in nitrogen metabolism in Streptococcus mutans.

2010-07-01 | GSE13540 | GEO

Proteomics of nitrogen metabolism in rhizosphere bacteria

Project description:In this study we use proteomics to study nitrogen metabolism in three taxonomically diverse bacterial strains previously isolated from Arabidopsis roots. The strains were cultivated on five different nitrogen sources (ammonium, glutamate, lysine, serine and urea), and label-free quantitative proteomics was used to profile cellular protein composition.

2020-03-19 | PXD011436 | Pride

A transcriptional-level gene regulation strategy in Chinese fir seedlings under nitrogen deficiency

Project description:To screen the genes related to nitrogen use efficiency, a Chinese fir clone named X6 was subjected to low nitrogen (LN) stress and was used along with control (CK) plants for de novo RNA sequencing of root transcriptomes. The differences in the expression of genes under both conditions were determined. A total of 4,300 significant differentially expressed genes (SDEGs) were obtained by comparing the transcriptomes of the plants in the LN and CK groups. Among these genes, 2,970 were upregulated, and 1,330 were downregulated. All the SDEGs obtained from the LN and CK samples were subjected to KEGG annotation and classification. Using classification and statistical analysis with WEGO software, KEGG pathway annotations were obtained for 980 SDEGs, which were related to 222 metabolic pathways, and the pathway for nitrogen metabolism was determined. Twenty-four unigenes related to nitrogen metabolism in the Chinese fir were screened. They were found to encode seven enzymes: nitrate reductase (NADH, NR1), ferredoxin-nitrite reductase (NIR1), glutamine synthetase (GS), glutamate synthase (ferredoxin; Fd-GOGAT), glutamate synthase (NADPH/NADH, NADH-GOGAT), glutamate dehydrogenase (NADP+, GDH1), and glutamate dehydrogenase (NAD(P)+,GDH2), as well as two transporters, namely, ammonium transporter (AMT) and nitrate transporter (NRT). Under nitrogen-deficient conditions, AMT, NRT, NR1, and GS were significantly upregulated, whereas NIR1, NADH-GOGAT, and GDH2 were significantly downregulated; the other genes showed no significant changes. This is the first report on the global transcriptome response of the Chinese fir to nitrogen deficiency. Our results might help illustrate the preference of the Chinese fir for NH4+ rather than for NO3−. Our data provide insights into the coordinated system of nitrogen metabolism in this valuable tree species.

2019-04-03 | GSE117392 | GEO