Goat embryo
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA422362 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Our data revealed the first miRNA profile related to the biology of the goat receptive endometrium during embryo implantation. The results suggested that a subset of miRNAs might play important roles in the formation of endometrial receptivity. Thus, elucidating the physiological roles of endometrial miRNAs will help us better understand the genetic control of implantation, which is the gateway to a successful pregnancy.
2017-02-09 | GSE63422 | GEO
Project description:Purpose: The goals of this study are to compare the differentially expressed genes in IFNT-treated and untreated goat endometrial epithelial cells and screen the signaling pathways related to endometrial receptivity. Methods: We performed RNA sequencing of goat endometrial epithelial cells (gEECs) with or without 20 ng/mL IFNT treatment. KEGG enrichment analysis was used to screen for enrichment pathways, and qRT-PCR, western blotting and immunohistochemistry were used for in vivo and in vitro validation. Results: Differential comparison showed that there were 442 upregulated differentially expressed genes (DEGs) and 510 downregulated DEGs. Bioinformatic analyses revealed that DEGs were significantly enriched in immune-related functions or pathways. The results of qRT-PCR, western blotting and immunohistochemistry were consistent with those of RNA-seq. Conclusion: These results highlight the role of IFNT in regulating endometrial receptivity in gEECs and uncover the temporal and spatial changes in the expression of STAT1/3 during embryo implantation in the goat endometrium.
2021-09-17 | GSE184110 | GEO
Project description:YTHDF2 regulates maternal transcriptome degradation and embryo development in goat
| PRJNA659520 | ENA
Project description:To explore functional circRNAs during goat muscle development, we systematically investigated the circRNAs profiles using high throughput transcriptome sequencing technology (RNA-seq) at key developmental stages of fetus and Kid in Haimen goat.
2023-12-31 | GSE200971 | GEO
Project description:Local breeds retained unique genetic variability important for adaptive potential especially in light of challenges related to climate change. Our objective was to perform, for the first time, a genome-wide diversity characterization using Illumina GoatSNP50 BeadChip of autochthonous Drežnica goat breed from Slovenia. Genetic diversity analyses revealed that the Slovenian Drežnica goat has a distinct genetic identity and is closely related to the neighboring Austrian and Italian alpine breeds. These results expand our knowledge on phylogeny of goat breeds from easternmost part of the European Alps.
2021-06-05 | GSE176157 | GEO
Project description:Preimplantation embryonic development is a dynamic, complex and precise regulated process to govern embryonic development and maintain embryo viability. The complete dynamics transcriptional activities during preimplantation development in the important domestic species goat is not available. Here, we performed RNA sequencing to establish a comprehensive transcriptional profiling of goat in vivo matured oocytes and preimplantation embryos. We found that the embryonic genome activation (EGA) in goat occurs at 16-cell stages, not previously defined at 8-cell stage, and showed much later EGA process compared to all other mammalian species. A total of 6,482 genes were identified to be significantly differentially expressed across all consecutive developmental stage comparisons and the important signaling pathways involved in each development transition were revealed. Additionally, we identified genes that appear to be transcribed only in a specific stage of development. Using weighted gene co-expression network analysis, we found 9 stage-specific modules of co-expressed genes that can be used to represent the corresponding stage of development. Furthermore, we identified conserved key members (or hub genes) of the goat expressed gene networks. Their vast association with other embryonic genes suggests that they may have important regulatory roles in embryo development. Our cross-mammalian species transcriptomic comparisons demarcated both conserved and goat specific features of preimplantation development.
2020-03-01 | GSE129742 | GEO
Project description:Different doses of glucose were inused into dairy goat mammary gland. The mammary gland tissues were biopsied to analyze the changes of transcriptome responding to glucose infusion.
2022-01-31 | GSE127429 | GEO
Project description:Little information is known regarding the maternal mRNA clearance and mechanisms behind it in farm animals. In the present study, we determined 3362 differentially expressed genes as the maternal mRNAs in goat using RNA-seq. We further reported that knockdown of YTHDF2 led to decreased blastocyst rate and impaired the maternal mRNAs clearance by downregulation of decapping enzymes DCP1A and DCP2, and the de-adenlyase CNOT1 and CNOT11.
2020-08-27 | GSE156924 | GEO
Project description:Transcriptomic analysis of STAT1/3 in the goat endometrium during embryo implantation
| PRJNA763197 | ENA
Project description:We use high-throughput single cell RNA-seq to determine expression profile of IVF-2cell 、 IVF-8cell and SCNT-8cell embryos,and C1 and over3720. At present, the strand-specific library could not be generated for the analysis of goat embryo lncRNA because of the lack of available specific rRNA removal kits for goats. In this study, single-cell RNA-seq technology was used to analyze early embryonic transcripts, enrich the involving transcripts, and construct an common transcript library. For identifying transcripts expressed during the early goat embryonic stage, three cDNA libraries were established from early goat embryos at the three developmental stages, namely, two-cell (IVF-2c)、 eight-cell embryos (IVF-8c) and somatic cell nuclear transfer（SCNT-8c). The GFFs cells transfected with control (no-load) and recombinant vectors were collected, total RNA was extracted, and the quality of the RNA was determined.
2022-04-04 | GSE199933 | GEO