Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Replacing reprogramming factors with antibodies selected from autocrine antibody libraries

ABSTRACT: Replacing reprogramming factors with antibodies selected from autocrine antibody libraries

PROVIDER: PRJNA422396 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6376050.fastq.gz	Fastqsanger.gz
	SRR6376060.fastq.gz	Fastqsanger.gz
	SRR6376070.fastq.gz	Fastqsanger.gz
	SRR6376051.fastq.gz	Fastqsanger.gz
	SRR6376061.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 32

Similar Datasets

Replacing reprogramming factors with antibodies selected from autocrine antibody libraries

Project description:Identification of antibodies with reprogramming activity from combinatorial libraries

2018-12-14 | GSE108068 | GEO

Identification of variant distribution in ethanol-selected sRNA libraries

Project description:Identification of variant distribution in ethanol-selected sRNA libraries

| PRJNA515434 | ENA

Small RNA libraries

Project description:Small RNA libraries

| PRJNA478199 | ENA

Autocrine TGF-β1 drives tissue-specific differentiation and function of resident NK cells

Project description:Group 1 innate lymphoid cells (ILCs) encompass NK cells and ILC1s which have non-redundant roles in host protection against pathogens and cancer. Despite their circulating nature, NK cells can establish residency in selected tissues during ontogeny, forming a distinct functional subset. The mechanisms that initiate, maintain, and regulate the conversion of NK cells into tissue-resident NK (trNK) cells are currently not well understood. Here, we identify autocrine transforming growth factor-b (TGF-b) as a cell-autonomous driver for NK cell tissue-residency across multiple glandular tissues during development. Cell-intrinsic production of TGF-β was continuously required for the maintenance of trNK cells and synergized with Hobit to enhance cytotoxic function. Whereas autocrine TGF-β was redundant in tumors, our study revealed that NK cell-derived TGF-β allowed expansion of cytotoxic trNK cells during local infection with murine cytomegalovirus (MCMV) and contributed to viral control in the salivary gland. Collectively, our findings reveal tissue-specific regulation of trNK cell differentiation and function by autocrine TGF-β1, which is relevant for anti-viral immunity.

2024-11-21 | GSE282148 | GEO

miRNA profiling of MCF7 autocrine hGH

Project description:MicroRNAs are widely expressed in the normal pubertal mammary gland and orchestrate mammary gland development by regulating cell proliferation, differentiation, apoptosis, and metabolism. Although human Growth hormone(hGH) plays fundamental roles in normal mammary gland development and elevated autocrine hGH levels have been documented to contribute to breast cancer, whether hGH should influence the expression pattern and the functional roles of miRNAs in this context remain unknown.This study explores the effects of autocrine hGH on microRNA expression in MCF7 cell.

2014-12-31 | GSE58845 | GEO

Internalizing cancer antibodies from phage libraries selected on tumor cells and yeast-displayed tumor antigens.

Project description:A number of approaches have been utilized to generate antibodies to cancer cell surface receptors that can be used as potential therapeutics. A number of these therapeutic approaches, including antibody-drug conjugates, immunotoxins, and targeted nucleic acid delivery, require antibodies that not only bind receptor but also undergo internalization into the cell upon binding. We previously reported on the ability to generate cancer cell binding and internalizing antibodies directly from human phage antibody libraries selected for internalization into cancer cell lines. While a number of useful antibodies have been generated using this approach, limitations include the inability to direct the selections to specific antigens and to identify the antigen bound by the antibodies. Here we show that these limitations can be overcome by using yeast-displayed antigens known to be associated with a cell type to select the phage antibody output after several rounds of selection on a mammalian cell line. We used this approach to generate several human phage antibodies to yeast-displayed EphA2 and CD44. The antibodies bound both yeast-displayed and mammalian cell surface antigens, and were endocytosed upon binding to mammalian cells. This approach is generalizable to many mammalian cell surface proteins, results in the generation of functional internalizing antibodies, and does not require antigen expression and purification for antibody generation.

| S-EPMC4195448 | biostudies-literature

Inovirus M13

Project description:M13 bacteriophage random peptide phage display libraries selected for binding to serum antibodies

| PRJNA196935 | ENA

Mycoplasma pneumoniae transposon libraries

Project description:Mycoplasma pneumoniae transposon libraries

| PRJEB9842 | ENA

Chemotherapy selected 4T1 cells

Project description:Chemotherapy selected 4T1 cells

| PRJNA892046 | ENA

Genetics of antinuclear antibodies

Project description:Genetics of antinuclear antibodies