Project description:To characterize how symbiotic bacteria affect the lolecular and cellular mechanisms of epithelial homeostasis, human colonic Caco-2 cells were co-culture with Lactobacillus casei and Bifidobacterium breve.
Project description:To investigate the effect of bacterially secreted Theta toxin on lung cancer cells, we established co-culture of live S. typhimurium and spheroids of H460 and H1819 cell lines. Once the bacteria colonized inside the spheroids, we induced the expression of Theta toxin by the bacteria. We extracted RNA after 1 week of treatment.
2022-12-21 | GSE210560 | GEO
Project description:soil bacteria microbial diversity in rice- eel co-culture system
Project description:Microbes are an important trigger of chronic rhinosinusitis (CRS). Staphylococcus aureus is a CRS-related pathogen that disrupts the epithelial barrier and activates mucosal inflammation. Given the ineffectiveness of antimicrobial therapy in CRS, we explored a potential treatment based on the interaction of bacteria within the dysbiotic microenvironment of the nasal mucosa. Microbiome analyses of clinical isolates from CRS patients and healthy controls were employed to identify Corynebacterium striatum as a potential inhibitor of pathogenic S. aureus. A co-infection culture model using human nasal epithelial cells (hNECs) was constructed to investigate the inhibition of S. aureus-induced disruption of the host epithelium by C. striatum. Further analysis of the inhibition of S. aureus by C. striatum was carried out using a bacteria co-culture model.
Project description:Microbes are an important trigger of chronic rhinosinusitis (CRS). Staphylococcus aureus is a CRS-related pathogen that disrupts the epithelial barrier and activates mucosal inflammation. Given the ineffectiveness of antimicrobial therapy in CRS, we explored a potential treatment based on the interaction of bacteria within the dysbiotic microenvironment of the nasal mucosa. Microbiome analyses of clinical isolates from CRS patients and healthy controls were employed to identify Corynebacterium striatum as a potential inhibitor of pathogenic S. aureus. A co-infection culture model using human nasal epithelial cells (hNECs) was constructed to investigate the inhibition of S. aureus-induced disruption of the host epithelium by C. striatum. Further analysis of the inhibition of S. aureus by C. striatum was carried out using a bacteria co-culture model.