Project description:To characterize how symbiotic bacteria affect the lolecular and cellular mechanisms of epithelial homeostasis, human colonic Caco-2 cells were co-culture with Lactobacillus casei and Bifidobacterium breve.
Project description:To investigate the effect of bacterially secreted Theta toxin on lung cancer cells, we established co-culture of live S. typhimurium and spheroids of H460 and H1819 cell lines. Once the bacteria colonized inside the spheroids, we induced the expression of Theta toxin by the bacteria. We extracted RNA after 1 week of treatment.
Project description:We established the co-culture model of iPS cell-derived crypt-villus structural small intestine and lactic acid bacteria (LABs). Using this model, the effect of TNF-α, IFN-γ and LABs (Lactobacillus delbrueckii subsp. bulgaricus 2038 and Streptococcus thermophilus 1131) on the cells was evaluated.