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A Macrophage Colony-Stimulating Factor-Producing gamma-delta T Cell Subset Prevents Malarial Parasitemic Recurrence

ABSTRACT: A Macrophage Colony-Stimulating Factor-Producing gamma-delta T Cell Subset Prevents Malarial Parasitemic Recurrence

PROVIDER: PRJNA427346 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6413680_1.fastq.gz	Fastqsanger.gz
	SRR6413680_2.fastq.gz	Fastqsanger.gz
	SRR6413681_1.fastq.gz	Fastqsanger.gz
	SRR6413681_2.fastq.gz	Fastqsanger.gz
	SRR6413682_1.fastq.gz	Fastqsanger.gz

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A Macrophage Colony-Stimulating Factor-Producing gamma-delta T Cell Subset Prevents Malarial Parasitemic Recurrence

Project description:Despite evidence that gd T cells play an important role during malaria, their precise role remains unclear. During murine malaria induced by Plasmodium chabaudi infection and in human P. falciparum infection, we found that gd T cells expanded rapidly after resolution of acute parasitemia, in contrast to ab T cells that expanded at the acute stage and then declined. Single-cell sequencing showed that TRAV15N-1 gd T cells were clonally expanded in mice and had convergent complementarity-determining region 3 sequences. These gd T cells expressed specific cytokines, M-CSF, CCL5, CCL3, which are known to act on the myeloid compartment, indicating that this gd T cell subset may have distinct functions. Both gd T cells and M-CSF were necessary for preventing parasitemic recurrence. These findings point to an M-CSF-producing gd T cell subset that fulfills a specialized protective role in the later stage of malaria infection when ab T cells have declined.

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Project description:Despite evidence that ?? T cells play an important role during malaria, their precise role remains unclear. During murine malaria induced by Plasmodium chabaudi infection and in human P. falciparum infection, we found that ?? T cells expanded rapidly after resolution of acute parasitemia, in contrast to ?? T cells that expanded at the acute stage and then declined. Single-cell sequencing showed that TRAV15N-1 (V?6.3) ?? T cells were clonally expanded in mice and had convergent complementarity-determining region 3 sequences. These ?? T cells expressed specific cytokines, M-CSF, CCL5, CCL3, which are known to act on myeloid cells, indicating that this ?? T cell subset might have distinct functions. Both ?? T cells and M-CSF were necessary for preventing parasitemic recurrence. These findings point to an M-CSF-producing ?? T cell subset that fulfills a specialized protective role in the later stage of malaria infection when ?? T cells have declined.

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Transcription profiling time series of mouse bone marrow macrophages treated with macrophage colony stimulating factor or granulocyte colony stimulating factor

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