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Inhibition of aberrant DNA re-methylation improves development of cloned embryos (RRBS-Seq)

ABSTRACT: Inhibition of aberrant DNA re-methylation improves development of cloned embryos (RRBS-Seq)

PROVIDER: PRJNA428419 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6436780_1.fastq.gz	Fastqsanger.gz
	SRR6436780_2.fastq.gz	Fastqsanger.gz
	SRR6436790_1.fastq.gz	Fastqsanger.gz
	SRR6436790_2.fastq.gz	Fastqsanger.gz
	SRR6436800_1.fastq.gz	Fastqsanger.gz

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Inhibition of aberrant DNA re-methylation improves development of cloned embryos

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Inhibition of aberrant DNA re-methylation improves development of cloned embryos (RRBS-Seq)

Project description:Somatic cell nuclear transfer (SCNT) enables the genome of a differentiated somatic cell to be reprogrammed to totipotency. However, this process is extremely inefficient, and the underlying mechanism of the epigenetic rearrangements following SCNT remains largely unknown. Here, we generated a genome-wide DNA methylome of mouse SCNT preimplantation embryos. Surprisingly, we identified widespread re-methylated regions (rDMRs) in 2- to 4-cell stage cloned embryos, which caused mis-expression of genes and retrotransposons important for zygotic genome activation and embryo development. Knocking-down DNA methyltransferases can specifically rescue the re-methylation defects of SCNT embryos and evidently improve the poor developmental capacity of cloned embryos. In addition, inactivation of DNA methyltransferases combined with overexpression of histone demethylases led to a more significant reduction in DNA methylation as well as exhibited a synergistic enhancement effect on the full-term development of nuclear transfer embryos. Our study therefore reveals that aberrant re-methylation functions as an unavoidable barrier for SCNT embryo development, and that the removal of multiple epigenetic barriers would be a promising approach to achieve the highest cloning efficiency.

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Inhibition of aberrant DNA re-methylation improves development of cloned embryos (WGBS-Seq)

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Project description:Inhibition of aberrant DNA re-methylation improves development of cloned embryos (RNA-Seq data set 1)

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Inhibition of aberrant DNA re-methylation improves development of cloned embryos (RNA-Seq data set 2)

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DNA methylation abnormal accumulation during cloned TSCs maintaining [RRBS]

Project description:Trophoblast stem cells (TSCs) are derived from the trophoectoderm of blastocysts and maintain ability to self-renewal and differentiation. TSC is a good model to research placenta development in vitro. It will contribute to understanding and improving cloned placentomegaly to compare the transcription and methylation between cloned and natural fertilized embryos throughout TSC derivation process. In the present study, we used SCNT (NT) and SCNT with HDACi treatment (SNT) as cloned groups and natural fertilized (NF) embryos to derive TSCs and chosed 5-time points to peform RNA-seq and RRBS. We found only NT got a barriar in TSC maintenace and both cloned groups exhited abnormal accumulating DNA methylation and it might be resiponsible for some malformations of cloned placentas.

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DNA methylation abnormal accumulation during cloned TSCs maintaining [RRBS]

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