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Inhibition of aberrant DNA re-methylation improves development of cloned embryos (WGBS-Seq)

ABSTRACT: Inhibition of aberrant DNA re-methylation improves development of cloned embryos (WGBS-Seq)

PROVIDER: PRJNA477280 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7405380_1.fastq.gz	Fastqsanger.gz
	SRR7405380_2.fastq.gz	Fastqsanger.gz
	SRR7405390_1.fastq.gz	Fastqsanger.gz
	SRR7405390_2.fastq.gz	Fastqsanger.gz
	SRR7405381_1.fastq.gz	Fastqsanger.gz

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Inhibition of aberrant DNA re-methylation improves development of cloned embryos

Project description:Inhibition of aberrant DNA re-methylation improves development of cloned embryos

| PRJNA428351 | ENA

Inhibition of aberrant DNA re-methylation improves development of cloned embryos (WGBS-Seq)

Project description:Somatic cell nuclear transfer (SCNT) enables the genome of a differentiated somatic cell to be reprogrammed to totipotency. However, this process is extremely inefficient, and the underlying mechanism of the epigenetic rearrangements following SCNT remains largely unknown. Here, we generated a genome-wide DNA methylome of mouse SCNT preimplantation embryos. Surprisingly, we identified widespread re-methylated regions (rDMRs) in 2- to 4-cell stage cloned embryos, which caused mis-expression of genes and retrotransposons important for zygotic genome activation and embryo development. Knocking-down DNA methyltransferases can specifically rescue the re-methylation defects of SCNT embryos and evidently improve the poor developmental capacity of cloned embryos. In addition, inactivation of DNA methyltransferases combined with overexpression of histone demethylases led to a more significant reduction in DNA methylation as well as exhibited a synergistic enhancement effect on the full-term development of nuclear transfer embryos. Our study therefore reveals that aberrant re-methylation functions as an unavoidable barrier for SCNT embryo development, and that the removal of multiple epigenetic barriers would be a promising approach to achieve the highest cloning efficiency.

2018-08-14 | GSE116103 | GEO

Inhibition of aberrant DNA re-methylation improves development of cloned embryos (RRBS-Seq)

Project description:Inhibition of aberrant DNA re-methylation improves development of cloned embryos (RRBS-Seq)

| PRJNA428419 | ENA

Inhibition of aberrant DNA re-methylation improves development of cloned embryos (RRBS-Seq)

2018-08-14 | GSE108708 | GEO

Inhibition of aberrant DNA re-methylation improves development of cloned embryos (RNA-Seq data set 1)

Project description:Inhibition of aberrant DNA re-methylation improves development of cloned embryos (RNA-Seq data set 1)

| PRJNA428420 | ENA

Inhibition of aberrant DNA re-methylation improves development of cloned embryos (RNA-Seq data set 2)

Project description:Inhibition of aberrant DNA re-methylation improves development of cloned embryos (RNA-Seq data set 2)

| PRJNA477282 | ENA