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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [P6 iCLIP]

ABSTRACT: Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [P6 iCLIP]

PROVIDER: PRJNA429350 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6456436.fastq.gz	Fastqsanger.gz
	SRR6456437.fastq.gz	Fastqsanger.gz
	SRR6456438.fastq.gz	Fastqsanger.gz

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [P6 iCLIP]

Project description:Dazl (deleted in azoospermia like) is a member of the DAZ family of germ cell-restricted RNA binding proteins required for gametogenesis from worm to human. The direct RNA targets and functions of these essential proteins are poorly understood. Here, we generated high-resolution, transcriptome-wide maps of Dazl-RNA interactions in mouse testes. These maps provide important insights into the mechanism of Dazl recruitment to mRNA and reveal Dazl binding to thousands of mRNAs predominantly through sequence-specific interactions near the polyA tail. Using transgenic mice and fluorescence activated cell sorting (FACS), we isolated DAZL knockout germ cells and used RNA-Seq to identify mRNAs sensitive to DAZL-ablation. Intersecting the RNA-Seq and Dazl-RNA interaction datasets revealed that Dazl enhances expression of a subset of directly-bound transcripts, namely mRNAs for a network of essential cell cycle regulatory genes. Collectively, our integrative analysis delineates a Dazl-dependent post-transcriptional gene regulatory program essential for mammalian germ cell maintenance.

2018-05-22 | GSE108183 | GEO

Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult PolyA-Seq]

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Spermatogonia PolyA-Seq]

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult PolyA-Seq]

Project description:Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult PolyA-Seq]

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Spermatogonia PolyA-Seq]

Project description:Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Spermatogonia PolyA-Seq]

| PRJNA429366 | ENA

Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions

Project description:Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult HITS-CLIP]

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult RNA-Seq]

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iCLIP of Dazl in mouse embryonic stem cells

Project description:In this experiment we would like to identify the binding sites of Dazl on the transcriptome of mESCs. To do that, we carried out an iCLIP experiment using mESCs grown in 2iL condition.

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [GFP cells RNA-Seq]

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