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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions

ABSTRACT: Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions

PROVIDER: PRJNA429360 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6456430.fastq.gz	Fastqsanger.gz
	SRR6456440.fastq.gz	Fastqsanger.gz
	SRR6456431_1.fastq.gz	Fastqsanger.gz
	SRR6456431_2.fastq.gz	Fastqsanger.gz
	SRR6456422.fastq.gz	Fastqsanger.gz

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Spermatogonia PolyA-Seq]

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult PolyA-Seq]

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult HITS-CLIP]

Project description:Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult HITS-CLIP]

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult RNA-Seq]

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [GFP cells RNA-Seq]

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Spermatogonia PolyA-Seq]

Project description:Dazl (deleted in azoospermia like) is a member of the DAZ family of germ cell-restricted RNA binding proteins required for gametogenesis from worm to human. The direct RNA targets and functions of these essential proteins are poorly understood. Here, we generated high-resolution, transcriptome-wide maps of Dazl-RNA interactions in mouse testes. These maps provide important insights into the mechanism of Dazl recruitment to mRNA and reveal Dazl binding to thousands of mRNAs predominantly through sequence-specific interactions near the polyA tail. Using transgenic mice and fluorescence activated cell sorting (FACS), we isolated DAZL knockout germ cells and used RNA-Seq to identify mRNAs sensitive to DAZL-ablation. Intersecting the RNA-Seq and Dazl-RNA interaction datasets revealed that Dazl enhances expression of a subset of directly-bound transcripts, namely mRNAs for a network of essential cell cycle regulatory genes. Collectively, our integrative analysis delineates a Dazl-dependent post-transcriptional gene regulatory program essential for mammalian germ cell maintenance.

2018-05-22 | GSE108996 | GEO

Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult PolyA-Seq]

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [P6 iCLIP]

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult RNA-Seq]

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