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Expression profiles of Ramularia collo-cygni (strain urug 2) grown on 6 conditions revealed common and different sets of regulated genes

ABSTRACT: Expression profiles of Ramularia collo-cygni (strain urug 2) grown on 6 conditions revealed common and different sets of regulated genes

PROVIDER: PRJNA429749 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6467450_1.fastq.gz	Fastqsanger.gz
	SRR6467450_2.fastq.gz	Fastqsanger.gz
	SRR6467451_1.fastq.gz	Fastqsanger.gz
	SRR6467451_2.fastq.gz	Fastqsanger.gz
	SRR6467446_1.fastq.gz	Fastqsanger.gz

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Ramularia collo-cygni

Project description:Ramularia collo-cygni overview

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Barley leaf infection with Ramularia collo-cygni

Project description:Barley plants (cv Fairytale and Tripple) were infected with two Ramularia collo-cygni isolates. Either DK05 or NZ11. Transcript and protein profiles were compared.

2024-01-24 | PXD039541 | Pride

Expression profiles of Ramularia collo-cygni (strain urug 2) grown on 6 conditions revealed common and different sets of regulated genes

Project description:The fungal RNA from 6 different conditions, especially one that mimics the plant environment (Barley Straw Agar –BSagar) was also sequenced to help in one hand the annotation and in other hand to uncover putative gene of interest that might be involved in the pathogenicity or the fungicide resistances for example.

2018-01-13 | GSE109135 | GEO

Ramularia collo-cygni genome, DK05

Project description:De novo genome assembly and comparative genomics of the newly important barley pathogen Ramularia collo-cygni.

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The genome sequence of Ramularia colly cygni URUG2

Project description:The genome sequence of Ramularia colly cygni URUG2, the barley pathogen causing RLS

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Fungicide Resistance Evolving in Ramularia collo-cygni Population in Estonia.

Project description:Ramularia leaf spot caused by the fungus Ramularia collo-cygni, has recently become widespread in Estonian barley fields. Currently, disease control in barley fields relies on SDHI and DMI fungicides, which might be threatened by R. collo-cygni isolates that are well-adapted to fungicide pressure. In a two-year study, 353 R. collo-cygni isolates were collected from spring barley fields in Estonia. A total of 153 R. collo-cygni isolates were examined for sensitivity to azoles (DMIs; prothioconazole-desthio, epoxiconazole, mefentrifluconazole) and succinate dehydrogenase inhibitors (SDHIs; boscalid, fluxapyroxad). Epoxiconazole was the least effective and a new fungicide mefentrifluconazole was the most effective DMI. Among SDHIs, fluxapyroxad was more effective than boscalid. Also, single R. collo-cygni isolates with high resistance to tested fungicides occurred, which could affect fungicide control of the pathogen. The entire collection of R. collo-cygni was analysed for mutations in fungicide target proteins. Six mutations were identified in CYP51 gene, the most dominant being I381T, I384T, and S459C. Also, numerous point mutations in the SdhC gene were present. The mutation G143A in strobilurin target protein CytB dominates in over 80% of the R. collo-cygni population, confirming the low efficacy of strobilurin fungicides in barley disease control.

| S-EPMC8307248 | biostudies-literature

The genome of the emerging barley pathogen Ramularia collo-cygni.

Project description:BackgroundRamularia collo-cygni is a newly important, foliar fungal pathogen of barley that causes the disease Ramularia leaf spot. The fungus exhibits a prolonged endophytic growth stage before switching life habit to become an aggressive, necrotrophic pathogen that causes significant losses to green leaf area and hence grain yield and quality.ResultsThe R. collo-cygni genome was sequenced using a combination of Illumina and Roche 454 technologies. The draft assembly of 30.3 Mb contained 11,617 predicted gene models. Our phylogenomic analysis confirmed the classification of this ascomycete fungus within the family Mycosphaerellaceae, order Capnodiales of the class Dothideomycetes. A predicted secretome comprising 1053 proteins included redox-related enzymes and carbohydrate-modifying enzymes and proteases. The relative paucity of plant cell wall degrading enzyme genes may be associated with the stealth pathogenesis characteristic of plant pathogens from the Mycosphaerellaceae. A large number of genes associated with secondary metabolite production, including homologs of toxin biosynthesis genes found in other Dothideomycete plant pathogens, were identified.ConclusionsThe genome sequence of R. collo-cygni provides a framework for understanding the genetic basis of pathogenesis in this important emerging pathogen. The reduced complement of carbohydrate-degrading enzyme genes is likely to reflect a strategy to avoid detection by host defences during its prolonged asymptomatic growth. Of particular interest will be the analysis of R. collo-cygni gene expression during interactions with the host barley, to understand what triggers this fungus to switch from being a benign endophyte to an aggressive necrotroph.

| S-EPMC4979122 | biostudies-literature

Deciphering Molecular Host-Pathogen Interactions During <i>Ramularia Collo-Cygni</i> Infection on Barley.

Project description:Ramularia collo-cygni is the causal agent of Ramularia leaf spot disease (RLS) on barley and became, during the recent decades, an increasing threat for farmers across the world. Here, we analyze morphological, transcriptional, and metabolic responses of two barley cultivars having contrasting tolerance to RLS, when infected by an aggressive or mild R. collo-cygni isolate. We found that fungal biomass in leaves of the two cultivars does not correlate with their tolerance to RLS, and both cultivars displayed cell wall reinforcement at the point of contact with the fungal hyphae. Comparative transcriptome analysis identified that the largest transcriptional differences between cultivars are at the early stages of fungal colonization with differential expression of kinases, calmodulins, and defense proteins. Weighted gene co-expression network analysis identified modules of co-expressed genes, and hub genes important for cultivar responses to the two R. collo-cygni isolates. Metabolite analyses of the same leaves identified defense compounds such as p-CHDA and serotonin, correlating with responses observed at transcriptome and morphological level. Together these all-round responses of barley to R. collo-cygni provide molecular tools for further development of genetic and physiological markers that may be tested for improving tolerance of barley to this fungal pathogen.

| S-EPMC8570322 | biostudies-literature

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